Overview

  • Product name

    Anti-VASP (phospho S157) antibody
    See all VASP primary antibodies
  • Description

    Rabbit polyclonal to VASP (phospho S157)
  • Host species

    Rabbit
  • Specificity

    Detects endogenous levels of VASP only when phosphorylated at serine 157.
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from Human VASP around the phosphorylation site of serine 157 (R-V-SP-N-A).

  • Positive control

    • Human tonsil tissue. NIH/3T3 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride

    Without Mg+2 and Ca+2
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorlyation site.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab47268 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 42 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/4000.

Target

  • Function

    Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.
  • Tissue specificity

    Highly expressed in platelets.
  • Sequence similarities

    Belongs to the Ena/VASP family.
    Contains 1 WH1 domain.
  • Domain

    The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.
    The WH1 domain mediates interaction with XIRP1.
  • Post-translational
    modifications

    Major substrate for cAMP-dependent (PKA) and cGMP-dependent protein kinase (PKG) in platelets. The preferred site for PKA is Ser-157, the preferred site for PKG, Ser-239. In ADP-activated platelets, phosphorylation by PKA or PKG on Ser-157 leads to fibrinogen receptor inhibition. Phosphorylation on Thr-278 requires prior phosphorylation on Ser-157 and Ser-239. In response to phorbol ester (PMA) stimulation, phosphorylated by PKC/PRKCA. In response to thrombin, phosphorylated by both PKC and ROCK1. Phosphorylation at Thr-278 by AMPK does not require prior phosphorylation at Ser-157 or Ser-239. Phosphorylation modulates F-actin binding, actin filament elongation and platelet activation. Carbon monoxide (CO) promotes phosphorylation at Ser-157, while nitric oxide (NO) promotes phosphorylation at Ser-157, but also at Ser-239. Response to NO and CO is blunted in platelets from diabetic patients, and VASP is not phosphorylated efficiently at Ser-157 and Ser-239.
  • Cellular localization

    Cytoplasm. Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Cell projection > lamellipodium membrane. Cell projection > filopodium membrane. Targeted to stress fibers and focal adhesions through interaction with a number of proteins including MRL family members. Localizes to the plasma membrane in protruding lamellipodia and filopodial tips. Stimulation by thrombin or PMA, also translocates VASP to focal adhesions. Localized along the sides of actin filaments throughout the peripheral cytoplasm under basal conditions.
  • Information by UniProt
  • Database links

  • Alternative names

    • Vasodilator stimulated phosphoprotein antibody
    • Vasodilator-stimulated phosphoprotein antibody
    • VASP antibody
    • VASP_HUMAN antibody

Images

  • ab47268, at 1/50 dilution, staining VASP in Human tonsil tissue by Immunohistochemistry, Paraffin-embedded tissue. Left panel: no peptide; right panel: with immunizing phosphopeptide.
  • All lanes : Anti-VASP (phospho S157) antibody (ab47268)

    Lane 1 : NIH/3T3 cell extract treated with forskolin (40µM, 30 min)
    Lane 2 : NIH/3T3 cell extract

    Predicted band size: 42 kDa

References

ab47268 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (platelet)
Loading amount
10 µg
Specification
platelet
Treatment
DMSO(negative control), 2mM cGMP analogue, 0.5 mM cAMP analogue (cBIMPS)
Gel Running Conditions
Reduced Denaturing (7.5 % gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 10 minute(s) · Concentration: 3% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Apr 06 2009

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (platelet)
Specification
platelet
Fixative
Formaldehyde
Permeabilization
Yes - 0.2% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Mar 25 2009

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