Anti-VASP (phospho S157) antibody (ab47268)
Key features and details
- Rabbit polyclonal to VASP (phospho S157)
- Suitable for: ELISA, ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-VASP (phospho S157) antibody
See all VASP primary antibodies -
Description
Rabbit polyclonal to VASP (phospho S157) -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Human VASP aa 100-200 (phospho S157).
Database link: P50552 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorlyation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab47268 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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ICC/IF | (1) |
Use at an assay dependent concentration.
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 42 kDa).
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 42 kDa). |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation. -
Tissue specificity
Highly expressed in platelets. -
Sequence similarities
Belongs to the Ena/VASP family.
Contains 1 WH1 domain. -
Domain
The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.
The WH1 domain mediates interaction with XIRP1. -
Post-translational
modificationsMajor substrate for cAMP-dependent (PKA) and cGMP-dependent protein kinase (PKG) in platelets. The preferred site for PKA is Ser-157, the preferred site for PKG, Ser-239. In ADP-activated platelets, phosphorylation by PKA or PKG on Ser-157 leads to fibrinogen receptor inhibition. Phosphorylation on Thr-278 requires prior phosphorylation on Ser-157 and Ser-239. In response to phorbol ester (PMA) stimulation, phosphorylated by PKC/PRKCA. In response to thrombin, phosphorylated by both PKC and ROCK1. Phosphorylation at Thr-278 by AMPK does not require prior phosphorylation at Ser-157 or Ser-239. Phosphorylation modulates F-actin binding, actin filament elongation and platelet activation. Carbon monoxide (CO) promotes phosphorylation at Ser-157, while nitric oxide (NO) promotes phosphorylation at Ser-157, but also at Ser-239. Response to NO and CO is blunted in platelets from diabetic patients, and VASP is not phosphorylated efficiently at Ser-157 and Ser-239. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Cell projection > lamellipodium membrane. Cell projection > filopodium membrane. Targeted to stress fibers and focal adhesions through interaction with a number of proteins including MRL family members. Localizes to the plasma membrane in protruding lamellipodia and filopodial tips. Stimulation by thrombin or PMA, also translocates VASP to focal adhesions. Localized along the sides of actin filaments throughout the peripheral cytoplasm under basal conditions. - Information by UniProt
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Database links
- Entrez Gene: 7408 Human
- Entrez Gene: 22323 Mouse
- Entrez Gene: 361517 Rat
- Omim: 601703 Human
- SwissProt: P50552 Human
- SwissProt: P70460 Mouse
- Unigene: 515469 Human
- Unigene: 9684 Mouse
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Alternative names
- Vasodilator stimulated phosphoprotein antibody
- Vasodilator-stimulated phosphoprotein antibody
- VASP antibody
- VASP_HUMAN antibody
Images
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ab47268, at 1/50 dilution, staining VASP in Human tonsil tissue by Immunohistochemistry, Paraffin-embedded tissue. Left panel: no peptide; right panel: with immunizing phosphopeptide.
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All lanes : Anti-VASP (phospho S157) antibody (ab47268)
Lane 1 : NIH/3T3 cell extract treated with forskolin (40µM, 30 min)
Lane 2 : NIH/3T3 cell extract
Predicted band size: 42 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab47268 has been referenced in 2 publications.
- Wang L et al. Asiaticoside produces an antidepressant-like effect in a chronic unpredictable mild stress model of depression in mice, involving reversion of inflammation and the PKA/pCREB/BDNF signaling pathway. Mol Med Rep 22:2364-2372 (2020). PubMed: 32705202
- Maass PG et al. PDE3A mutations cause autosomal dominant hypertension with brachydactyly. Nat Genet 47:647-53 (2015). PubMed: 25961942