Product nameAnti-Vaspin antibody [EPR23079-196] - BSA and Azide free
See all Vaspin primary antibodies
DescriptionRabbit monoclonal [EPR23079-196] to Vaspin - BSA and Azide free
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse
Recombinant fragment within Mouse Vaspin aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q7TMF5
- WB: Mouse skin and stomach tissue lysates; His-tagged recombinant mouse Vaspin protein (aa 20-413). IP: Mouse stomach tissue lysate.
This antibody (Clone EPR23079-196) is a different clone to ab267471 (Clone EPR23080-232).
ab273096 is the carrier-free version of ab267470. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab273096 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.??
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab273096 in the following tested applications.
|WB||Use at an assay dependent concentration. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).|
|IP||Use at an assay dependent concentration.|
FunctionMay modulates insulin action conceivably only in the presence of its yet undefined target proteases in white adipose tissues.
Tissue specificityExpressed in visceral adipose tissues.
Sequence similaritiesBelongs to the serpin family.
- Information by UniProt
- ENSG00000165953 antibody
- OL 64 antibody
- OL-64 antibody
Vaspin was immunoprecipitated from 0.35 mg Mouse stomach tissue lysate 10 with ab267470 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab267470 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.
Lane 1: Mouse stomach tissue lysate 10 ug
Lane 2: ab267470 IP in Mouse stomach tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 24 seconds
Vaspin protein can form polymers when heated as described in the literature (PMID: 28668641).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267470).
ab273096 has not yet been referenced specifically in any publications.