Recombinant Anti-Vaspin antibody [EPR23080-232] (ab267471)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23080-232] to Vaspin
- Suitable for: IP, WB
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Vaspin antibody [EPR23080-232]
See all Vaspin primary antibodies -
Description
Rabbit monoclonal [EPR23080-232] to Vaspin -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details
Unsuitable for: IHC-Fr or IHC-P -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Rat stomach and Rat skin lysate; His-tagged recombinant Rat Serpin A12 Protein. IP: Rat stomach tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23080-232 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab267471 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
1/30.
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WB |
1/1000. Predicted molecular weight: 47 kDa.
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Notes |
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IP
1/30. |
WB
1/1000. Predicted molecular weight: 47 kDa. |
Target
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Function
May modulates insulin action conceivably only in the presence of its yet undefined target proteases in white adipose tissues. -
Tissue specificity
Expressed in visceral adipose tissues. -
Sequence similarities
Belongs to the serpin family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 145264 Human
- Entrez Gene: 191570 Rat
- SwissProt: Q8IW75 Human
- SwissProt: Q8R4Z1 Rat
- Unigene: 99476 Human
- Unigene: 79035 Rat
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Alternative names
- ENSG00000165953 antibody
- OL 64 antibody
- OL-64 antibody
see all
Images
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All lanes : Anti-Vaspin antibody [EPR23080-232] (ab267471) at 1/1000 dilution
Lane 1 : Rat stomach tissue lysate at 20 µg
Lane 2 : Rat kidney tissue lysate at 20 µg
Lane 3 : Rat skin tissue lysate at 60 µg
Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 47 kDa
Observed band size: 42,47 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The band of 42kDa is the endogenously cleaved form.
The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 19554505, 16030142, 26199422).
Negative control: kidney (PMID: 19554505).
Exposure time: lane1-2: 59 seconds lane 3: 3 minutes
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Vaspin was immunoprecipitated from 0.35 mg Rat stomach tissue lysate with ab267471 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab267471 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Rat stomach tissue lysate 10ug
Lane 2: ab267471 IP in Rat stomach tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab267471 in Rat stomach tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes
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Anti-Vaspin antibody [EPR23080-232] (ab267471) at 1/1000 dilution + His-tagged recombinant Rat Serpin A12 Protein (aa20-411),
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 26 seconds
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab267471 has not yet been referenced specifically in any publications.