Anti-VAT1 antibody (ab242045)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-VAT1 antibody
See all VAT1 primary antibodies -
Description
Rabbit polyclonal to VAT1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human VAT1 aa 1-50. The exact sequence is proprietary.
Database link: Q99536 -
Positive control
- WB: HeLa, Jurkat and HEK-293T whole cell lysate. IP: VAT1 IP in HEK-293T whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to VAT1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab242045 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-10 µg/mg of lysate. | |
| WB | 1/2000 - 1/10000. |
Target
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Function
Possesses ATPase activity (By similarity). Plays a part in calcium-regulated keratinocyte activation in epidermal repair mechanisms. Has no effect on cell proliferation. -
Tissue specificity
Expressed in brain. Also expressed in glioblastoma cells. -
Sequence similarities
Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 10493 Human
- Omim: 604631 Human
- SwissProt: Q99536 Human
- Unigene: 514199 Human
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Alternative names
- VAT1 antibody
- FLJ20230 antibody
- Membrane protein of cholinergic synaptic vesicles antibody
see all
Images
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Western blot - Anti-VAT1 antibody (ab242045)All lanes : Anti-VAT1 antibody (ab242045) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Exposure time: 10 secondsPrepared using NETN lysis buffer.
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Immunoprecipitation - Anti-VAT1 antibody (ab242045)VAT1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab242045 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab242045 at 0.4 µg/ml.
Lane 1: ab242045 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG in HEK-293T whole cell lysate.Detection: Chemiluminescence with an exposure time of 10 seconds.
Prepared using NETN lysis buffer.
Datasheets and documents
References
ab242045 has not yet been referenced specifically in any publications.
