Product nameAnti-Vav proteins antibody
See all Vav proteins primary antibodies
DescriptionRabbit polyclonal to Vav proteins
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide, corresponding to amino acids 172-176 of Human Vav proteins
- Jurkat or THP1 cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab92890 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000. Predicted molecular weight: 98 kDa (VAV1 & VAV3). 101 kDa (VAV2)
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceThe Vav family are Rho/Rac guanosine nucleotide exchange factors (GEFs), consisting of three members in mammalian cells (Vav, Vav2, Vav3) and one in nematodes (CelVav). First discovered based on its transforming properties, Vav is expressed mainly in hematopoietic cells and a few non-hematopoietic tissues, such as the pancreas and tooth enamels. As a signalling transducer, Vav is involved in T-cell activated transduction of T-cell antigen receptor (TCR). T-cell stimulated and tyrosine phosphorylated Vav acts as a catalyst in the exchange of guanosine nucleotides on Rac-1, a GTP binding protein. Using a mouse model, Vav expression has been determined to play an essential role in the cyctosketetal, proliferative, and apoptotic pathways for developing lymphoid cells and its signal response.
Cellular localizationCytoplasmic and Plasma membrane VAV1, Cytoplasm - VAV2 & VAV3
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Immunoprecipitation of VAV proteins from 500µg of Jurkat (Lane 1) and THP1 (Lane 2) cell lysates, using 2µg of ab92890.
All lanes : Anti-Vav proteins antibody (ab92890) at 2 µg
Lane 1 : Jurkat cell lysate
Lane 2 : THP1 cell lysate
Lysates/proteins at 500 µg per lane.
All lanes : Anti-Vav proteins antibody (ab92890) at 1/1000 dilution
Lane 1 : Jurkat cell lysate at 3 µg
Lane 2 : Jurkat cell lysate at 10 µg
Lane 3 : Jurkat cell lysate at 30 µg
Predicted band size: 98 kDa
ab92890 has not yet been referenced specifically in any publications.