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    vav-proteins-antibody-ep482y-ab40875.pdf

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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other
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RecombinantRabMAb

Recombinant Anti-Vav proteins antibody [EP482Y] (ab40875)

  • Datasheet
  • SDS
Reviews (6) Submit a question References (6)

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Western blot - Anti-Vav proteins antibody [EP482Y] (ab40875)
  • Flow Cytometry (Intracellular) - Anti-Vav proteins antibody [EP482Y] (ab40875)
  • Anti-Vav proteins antibody [EP482Y] (ab40875)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP482Y] to Vav proteins
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Protein
Product image
Recombinant Human Vav proteins (ab172184)
Isotype
Product image
Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (ab18413)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Vav proteins antibody [EP482Y]
    See all Vav proteins primary antibodies
  • Description

    Rabbit monoclonal [EP482Y] to Vav proteins
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Vav proteins aa 750-850 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Jurkat cells lysate
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA, 49% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP482Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Other
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family

Associated products

  • Alternative Versions

    • Anti-Vav proteins antibody [EP482Y] - BSA and Azide free (ab247298)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human Vav proteins (ab172184)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab40875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB (2)
1/2000. Predicted molecular weight: 98 kDa. Also 101 or 87 kDa, depending on the isoform.
Notes
Flow Cyt (Intra)
1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/2000. Predicted molecular weight: 98 kDa. Also 101 or 87 kDa, depending on the isoform.

Target

  • Relevance

    The Vav family are Rho/Rac guanosine nucleotide exchange factors (GEFs), consisting of three members in mammalian cells (Vav, Vav2, Vav3) and one in nematodes (CelVav). First discovered based on its transforming properties, Vav is expressed mainly in hematopoietic cells and a few non-hematopoietic tissues, such as the pancreas and tooth enamels. As a signalling transducer, Vav is involved in T-cell activated transduction of T-cell antigen receptor (TCR). T-cell stimulated and tyrosine phosphorylated Vav acts as a catalyst in the exchange of guanosine nucleotides on Rac-1, a GTP binding protein. Using a mouse model, Vav expression has been determined to play an essential role in the cyctosketetal, proliferative, and apoptotic pathways for developing lymphoid cells and its signal response.
  • Cellular localization

    Cytoplasmic and Plasma membrane VAV1, Cytoplasm - VAV2 & VAV3
  • Database links

    • Entrez Gene: 10451 Human
    • Entrez Gene: 7409 Human
    • Entrez Gene: 7410 Human
    • Omim: 164875 Human
    • Omim: 600428 Human
    • Omim: 605541 Human
    • SwissProt: P15498 Human
    • SwissProt: P52735 Human
    • SwissProt: Q9UKW4 Human
    see all
  • Alternative names

    • 2810040F13Rik antibody
    • A530094I06Rik antibody
    • AA986410 antibody
    • AI847175 antibody
    • FLJ40431 antibody
    • GDP/GTP exchange factor VAV2 antibody
    • Guanine nucleotide exchange factor VAV2 antibody
    • Guanine nucleotide exchange factor VAV3 antibody
    • MGC27838 antibody
    • Oncogene VAV antibody
    • Oncogene VAV1 antibody
    • Oncogene VAV2 antibody
    • Oncogene VAV3 antibody
    • p95 antibody
    • p95vav antibody
    • Protein vav 1 antibody
    • Protein vav 2 antibody
    • Protein vav 3 antibody
    • Proto oncogene vav antibody
    • RGD1565941 antibody
    • VAV antibody
    • vav 1 guanine nucleotide exchange factor antibody
    • Vav 1 oncogene antibody
    • vav 2 guanine nucleotide exchange factor antibody
    • Vav 2 oncogene antibody
    • vav 3 guanine nucleotide exchange factor antibody
    • Vav 3 oncogene antibody
    • vav-T antibody
    • VAV1 oncogene antibody
    • VAV2 oncogene antibody
    • VAV3 oncogene antibody
    see all

Images

  • Western blot - Anti-Vav proteins antibody [EP482Y] (ab40875)
    Western blot - Anti-Vav proteins antibody [EP482Y] (ab40875)
    Anti-Vav proteins antibody [EP482Y] (ab40875) at 1/2000 dilution + Jurkat cell lysate at 10 µg

    Predicted band size: 98 kDa
    Observed band size: 98 kDa

  • Flow Cytometry (Intracellular) - Anti-Vav proteins antibody [EP482Y] (ab40875)
    Flow Cytometry (Intracellular) - Anti-Vav proteins antibody [EP482Y] (ab40875)

    Overlay histogram showing Jurkat cells stained with ab40875 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40875, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

     

  • Anti-Vav proteins antibody [EP482Y] (ab40875)
    Anti-Vav proteins antibody [EP482Y] (ab40875)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (6)

Publishing research using ab40875? Please let us know so that we can cite the reference in this datasheet.

ab40875 has been referenced in 6 publications.

  • Bacchelli C  et al. Mutations in linker for activation of T cells (LAT) lead to a novel form of severe combined immunodeficiency. J Allergy Clin Immunol 139:634-642.e5 (2017). PubMed: 27522155
  • Gastaldello S  et al. Caspase-1 promotes Epstein-Barr virus replication by targeting the large tegument protein deneddylase to the nucleus of productively infected cells. PLoS Pathog 9:e1003664 (2013). Human . PubMed: 24130483
  • Pauker MH  et al. Studying the dynamics of SLP-76, Nck, and Vav1 multimolecular complex formation in live human cells with triple-color FRET. Sci Signal 5:rs3 (2012). IP . PubMed: 22534133
  • Zyss D  et al. Casein kinase I delta controls centrosome positioning during T cell activation. J Cell Biol 195:781-97 (2011). ICC/IF . PubMed: 22123863
  • Barda-Saad M  et al. Cooperative interactions at the SLP-76 complex are critical for actin polymerization. EMBO J 29:2315-28 (2010). PubMed: 20562827
  • Gianni D  et al. The involvement of the tyrosine kinase c-Src in the regulation of reactive oxygen species generation mediated by NADPH oxidase-1. Mol Biol Cell 19:2984-94 (2008). WB ; Human . PubMed: 18463161

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-6 of 6 Abreviews or Q&A

Western blot abreview for Anti-Vav proteins antibody [EP482Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Melanoma cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Melanoma cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 20 2021

Immunocytochemistry abreview for Anti-Vav proteins antibody [EP482Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cultured Cells (Melanoma cells)
Permeabilization
No
Specification
Melanoma cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 23°C
Fixative
Acetone
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 16 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Vav proteins antibody [EP482Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Skin (dermis, blood vessels))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Specification
Skin (dermis, blood vessels)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 29 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-Vav proteins antibody [EP482Y]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Melanoma cells)
Permeabilization
Yes - Triton
Specification
Melanoma cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Acetone
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 28 2020

Immunohistochemistry (Frozen sections) abreview for Anti-Vav proteins antibody [EP482Y]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Skin - Nevus)
Permeabilization
No
Specification
Skin - Nevus
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Acetone
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 14 2019

Western blot abreview for Anti-Vav proteins antibody [EP482Y]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (platelet)
Loading amount
25 µg
Specification
platelet
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 20 minute(s) · Concentration: 3% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 19 2011

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