Product nameAnti-Vav proteins antibody [EP482Y]
See all Vav proteins primary antibodies
DescriptionRabbit monoclonal [EP482Y] to Vav proteins
Tested applicationsSuitable for: WB, Flow Cyt, ICC/IF, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Vav proteins aa 750-850 (C terminal). The exact sequence is proprietary.
- Jurkat cells lysate
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA, 49% PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab40875 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 98 kDa. Also 101 or 87 kDa, depending on the isoform.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
RelevanceThe Vav family are Rho/Rac guanosine nucleotide exchange factors (GEFs), consisting of three members in mammalian cells (Vav, Vav2, Vav3) and one in nematodes (CelVav). First discovered based on its transforming properties, Vav is expressed mainly in hematopoietic cells and a few non-hematopoietic tissues, such as the pancreas and tooth enamels. As a signalling transducer, Vav is involved in T-cell activated transduction of T-cell antigen receptor (TCR). T-cell stimulated and tyrosine phosphorylated Vav acts as a catalyst in the exchange of guanosine nucleotides on Rac-1, a GTP binding protein. Using a mouse model, Vav expression has been determined to play an essential role in the cyctosketetal, proliferative, and apoptotic pathways for developing lymphoid cells and its signal response.
Cellular localizationCytoplasmic and Plasma membrane VAV1, Cytoplasm - VAV2 & VAV3
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Anti-Vav proteins antibody [EP482Y] (ab40875) at 1/2000 dilution + Jurkat cell lysate (10 ug).
Predicted band size: 98 kDa
Observed band size: 98 kDa
Overlay histogram showing Jurkat cells stained with ab40875 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40875, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
This product has been referenced in:
- Gastaldello S et al. Caspase-1 promotes Epstein-Barr virus replication by targeting the large tegument protein deneddylase to the nucleus of productively infected cells. PLoS Pathog 9:e1003664 (2013). Human . Read more (PubMed: 24130483) »
- Pauker MH et al. Studying the dynamics of SLP-76, Nck, and Vav1 multimolecular complex formation in live human cells with triple-color FRET. Sci Signal 5:rs3 (2012). IP . Read more (PubMed: 22534133) »