Recombinant Anti-Vav proteins antibody [EP482Y] (ab40875)
![Western blot - Anti-Vav proteins antibody [EP482Y] (ab40875)](https://www.abcam.com/ps/products/40/ab40875/Images/ab40875-54168-anti-vav-proteins-antibody-ep482y-western-blot.jpg "Western blot - Anti-Vav proteins antibody [EP482Y] (ab40875)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP482Y] to Vav proteins
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-Vav proteins antibody [EP482Y]
See all Vav proteins primary antibodies -
Description
Rabbit monoclonal [EP482Y] to Vav proteins -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Vav proteins aa 750-850 (C terminal). The exact sequence is proprietary.
-
Positive control
- Jurkat cells lysate
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA, 49% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP482Y -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab40875 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (2) |
1/2000. Predicted molecular weight: 98 kDa. Also 101 or 87 kDa, depending on the isoform.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/2000. Predicted molecular weight: 98 kDa. Also 101 or 87 kDa, depending on the isoform. |
Target
-
Relevance
The Vav family are Rho/Rac guanosine nucleotide exchange factors (GEFs), consisting of three members in mammalian cells (Vav, Vav2, Vav3) and one in nematodes (CelVav). First discovered based on its transforming properties, Vav is expressed mainly in hematopoietic cells and a few non-hematopoietic tissues, such as the pancreas and tooth enamels. As a signalling transducer, Vav is involved in T-cell activated transduction of T-cell antigen receptor (TCR). T-cell stimulated and tyrosine phosphorylated Vav acts as a catalyst in the exchange of guanosine nucleotides on Rac-1, a GTP binding protein. Using a mouse model, Vav expression has been determined to play an essential role in the cyctosketetal, proliferative, and apoptotic pathways for developing lymphoid cells and its signal response. -
Cellular localization
Cytoplasmic and Plasma membrane VAV1, Cytoplasm - VAV2 & VAV3 -
Database links
- Entrez Gene: 10451 Human
- Entrez Gene: 7409 Human
- Entrez Gene: 7410 Human
- Omim: 164875 Human
- Omim: 600428 Human
- Omim: 605541 Human
- SwissProt: P15498 Human
- SwissProt: P52735 Human
see all -
Alternative names
- 2810040F13Rik antibody
- A530094I06Rik antibody
- AA986410 antibody
see all
Images
-
Western blot - Anti-Vav proteins antibody [EP482Y] (ab40875)Anti-Vav proteins antibody [EP482Y] (ab40875) at 1/2000 dilution + Jurkat cell lysate at 10 µg
Predicted band size: 98 kDa
Observed band size: 98 kDa -
![Flow Cytometry (Intracellular) - Anti-Vav proteins antibody [EP482Y] (ab40875)](https://www.abcam.com/ps/products/40/ab40875/Images/ab40875-54167-anti-vav-proteins-antibody-ep482y-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Vav proteins antibody [EP482Y] (ab40875)Overlay histogram showing Jurkat cells stained with ab40875 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40875, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
-
![Anti-Vav proteins antibody [EP482Y] (ab40875)](https://www.abcam.com/ps/products/40/ab40875/Images/ab40875-3-benefits-of-recombinant-antibodies.png)
Anti-Vav proteins antibody [EP482Y] (ab40875)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (6)
ab40875 has been referenced in 6 publications.
- Bacchelli C et al. Mutations in linker for activation of T cells (LAT) lead to a novel form of severe combined immunodeficiency. J Allergy Clin Immunol 139:634-642.e5 (2017). PubMed: 27522155
- Gastaldello S et al. Caspase-1 promotes Epstein-Barr virus replication by targeting the large tegument protein deneddylase to the nucleus of productively infected cells. PLoS Pathog 9:e1003664 (2013). Human . PubMed: 24130483
- Pauker MH et al. Studying the dynamics of SLP-76, Nck, and Vav1 multimolecular complex formation in live human cells with triple-color FRET. Sci Signal 5:rs3 (2012). IP . PubMed: 22534133
- Zyss D et al. Casein kinase I delta controls centrosome positioning during T cell activation. J Cell Biol 195:781-97 (2011). ICC/IF . PubMed: 22123863
- Barda-Saad M et al. Cooperative interactions at the SLP-76 complex are critical for actin polymerization. EMBO J 29:2315-28 (2010). PubMed: 20562827
- Gianni D et al. The involvement of the tyrosine kinase c-Src in the regulation of reactive oxygen species generation mediated by NADPH oxidase-1. Mol Biol Cell 19:2984-94 (2008). WB ; Human . PubMed: 18463161