Anti-Vav proteins antibody [EP482Y] (ab40875)


  • Product name
    Anti-Vav proteins antibody [EP482Y]
    See all Vav proteins primary antibodies
  • Description
    Rabbit monoclonal [EP482Y] to Vav proteins
  • Host species
  • Tested applications
    Suitable for: WB, Flow Cyt, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Vav proteins aa 750-850 (C terminal). The exact sequence is proprietary.

  • Positive control
    • Jurkat cells lysate
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab40875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 98 kDa. Also 101 or 87 kDa, depending on the isoform.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/50.
IP 1/50.


  • Relevance
    The Vav family are Rho/Rac guanosine nucleotide exchange factors (GEFs), consisting of three members in mammalian cells (Vav, Vav2, Vav3) and one in nematodes (CelVav). First discovered based on its transforming properties, Vav is expressed mainly in hematopoietic cells and a few non-hematopoietic tissues, such as the pancreas and tooth enamels. As a signalling transducer, Vav is involved in T-cell activated transduction of T-cell antigen receptor (TCR). T-cell stimulated and tyrosine phosphorylated Vav acts as a catalyst in the exchange of guanosine nucleotides on Rac-1, a GTP binding protein. Using a mouse model, Vav expression has been determined to play an essential role in the cyctosketetal, proliferative, and apoptotic pathways for developing lymphoid cells and its signal response.
  • Cellular localization
    Cytoplasmic and Plasma membrane VAV1, Cytoplasm - VAV2 & VAV3
  • Database links
  • Alternative names
    • 2810040F13Rik antibody
    • A530094I06Rik antibody
    • AA986410 antibody
    • AI847175 antibody
    • FLJ40431 antibody
    • GDP/GTP exchange factor VAV2 antibody
    • Guanine nucleotide exchange factor VAV2 antibody
    • Guanine nucleotide exchange factor VAV3 antibody
    • MGC27838 antibody
    • Oncogene VAV antibody
    • Oncogene VAV1 antibody
    • Oncogene VAV2 antibody
    • Oncogene VAV3 antibody
    • p95 antibody
    • p95vav antibody
    • Protein vav 1 antibody
    • Protein vav 2 antibody
    • Protein vav 3 antibody
    • Proto oncogene vav antibody
    • RGD1565941 antibody
    • VAV antibody
    • vav 1 guanine nucleotide exchange factor antibody
    • Vav 1 oncogene antibody
    • vav 2 guanine nucleotide exchange factor antibody
    • Vav 2 oncogene antibody
    • vav 3 guanine nucleotide exchange factor antibody
    • Vav 3 oncogene antibody
    • vav-T antibody
    • VAV1 oncogene antibody
    • VAV2 oncogene antibody
    • VAV3 oncogene antibody
    see all


  • Anti-Vav proteins antibody [EP482Y] (ab40875) at 1/2000 dilution + Jurkat cell lysate (10 ug).

    Predicted band size: 98 kDa
    Observed band size: 98 kDa

  • Overlay histogram showing Jurkat cells stained with ab40875 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40875, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.


This product has been referenced in:
  • Gastaldello S  et al. Caspase-1 promotes Epstein-Barr virus replication by targeting the large tegument protein deneddylase to the nucleus of productively infected cells. PLoS Pathog 9:e1003664 (2013). Human . Read more (PubMed: 24130483) »
  • Pauker MH  et al. Studying the dynamics of SLP-76, Nck, and Vav1 multimolecular complex formation in live human cells with triple-color FRET. Sci Signal 5:rs3 (2012). IP . Read more (PubMed: 22534133) »

See all 5 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (platelet)
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 20 minute(s) · Concentration: 3% · Temperature: 23°C

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Verified customer

Submitted Aug 19 2011


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