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  1. Link

    vav1-antibody-ab245440.pdf

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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Regulators
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Anti-VAV1 antibody (ab245440)

  • Datasheet
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Western blot - Anti-VAV1 antibody (ab245440)
  • Immunoprecipitation - Anti-VAV1 antibody (ab245440)

Key features and details

  • Rabbit polyclonal to VAV1
  • Suitable for: IP, WB
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human VAV1 protein (ab176044)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-VAV1 antibody
    See all VAV1 primary antibodies
  • Description

    Rabbit polyclonal to VAV1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human VAV1 aa 175-225. The exact sequence is proprietary.
    Database link: P15498

  • Positive control

    • WB: Jurkat whole cell lysate. IP: VAV1 IP in Jurkat whole cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245440 was affinity purified using an epitope specific to VAV1 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Regulators
    • Cell Biology
    • Cell Cycle
    • Cell differentiation
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Cancer
    • Signal transduction
    • G protein signaling
    • Small G proteins
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Positive Controls

    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human VAV1 protein (ab176044)

Applications

Our Abpromise guarantee covers the use of ab245440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.

20 µl/mg lysate

WB 1/1000.

Target

  • Function

    Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.
  • Tissue specificity

    Widely expressed in hematopoietic cells but not in other cell types.
  • Sequence similarities

    Contains 1 CH (calponin-homology) domain.
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 SH2 domain.
    Contains 2 SH3 domains.
  • Domain

    The DH domain is involved in interaction with CCPG1.
  • Post-translational
    modifications

    Phosphorylated on tyrosine residues.
  • Target information above from: UniProt accession P15498 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7409 Human
    • Omim: 164875 Human
    • SwissProt: P15498 Human
    • Unigene: 116237 Human
    • Alternative names

      • Oncogene vav antibody
      • p95Vav antibody
      • Proto-oncogene vav antibody
      • Protooncogene vav antibody
      • VAV 1 antibody
      • VAV 1 oncogene antibody
      • VAV antibody
      • Vav proto oncogene antibody
      • VAV_HUMAN antibody
      • VAV1 antibody
      see all

    Images

    • Western blot - Anti-VAV1 antibody (ab245440)
      Western blot - Anti-VAV1 antibody (ab245440)
      All lanes : Anti-VAV1 antibody (ab245440) at 1/1000 dilution

      Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

      Lysates/proteins at 15 µg per lane.

      Exposure time: 30 seconds


      Prepared using NETN lysis buffer.

    • Immunoprecipitation - Anti-VAV1 antibody (ab245440)
      Immunoprecipitation - Anti-VAV1 antibody (ab245440)

      VAV1 was immunoprecipitated from Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
      ab245440 used for IP at 20 µl per reaction. For WB at a 1/250 dilution.

      Lane 1: ab245440 IP in Jurkat whole cell lysate.
      Lane 2: Control IgG in Jurkat whole cell lysate.
      Chemiluminescence detection: 30 seconds.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab245440? Please let us know so that we can cite the reference in this datasheet.

    ab245440 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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