Key features and details
- Rabbit polyclonal to VAV1
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-VAV1 antibody
See all VAV1 primary antibodies
DescriptionRabbit polyclonal to VAV1
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human VAV1 aa 175-225. The exact sequence is proprietary.
Database link: P15498
- WB: Jurkat whole cell lysate. IP: VAV1 IP in Jurkat whole cell lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245440 was affinity purified using an epitope specific to VAV1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245440 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
20 µl/mg lysate
FunctionCouples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.
Tissue specificityWidely expressed in hematopoietic cells but not in other cell types.
Sequence similaritiesContains 1 CH (calponin-homology) domain.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 SH2 domain.
Contains 2 SH3 domains.
DomainThe DH domain is involved in interaction with CCPG1.
modificationsPhosphorylated on tyrosine residues.
- Information by UniProt
- Oncogene vav antibody
- p95Vav antibody
- Proto-oncogene vav antibody
All lanes : Anti-VAV1 antibody (ab245440) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 15 µg per lane.
Exposure time: 30 seconds
Prepared using NETN lysis buffer.
VAV1 was immunoprecipitated from Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab245440 used for IP at 20 µl per reaction. For WB at a 1/250 dilution.
Lane 1: ab245440 IP in Jurkat whole cell lysate.
Lane 2: Control IgG in Jurkat whole cell lysate.
Chemiluminescence detection: 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245440 has not yet been referenced specifically in any publications.