Anti-VAV1 antibody (ab245440)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-VAV1 antibody
See all VAV1 primary antibodies -
Description
Rabbit polyclonal to VAV1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human VAV1 aa 175-225. The exact sequence is proprietary.
Database link: P15498 -
Positive control
- WB: Jurkat whole cell lysate. IP: VAV1 IP in Jurkat whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245440 was affinity purified using an epitope specific to VAV1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab245440 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent concentration. 20 µl/mg lysate |
|
| WB | 1/1000. |
Target
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Function
Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation. -
Tissue specificity
Widely expressed in hematopoietic cells but not in other cell types. -
Sequence similarities
Contains 1 CH (calponin-homology) domain.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 SH2 domain.
Contains 2 SH3 domains. -
Domain
The DH domain is involved in interaction with CCPG1. -
Post-translational
modificationsPhosphorylated on tyrosine residues. - Information by UniProt
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Database links
- Entrez Gene: 7409 Human
- Omim: 164875 Human
- SwissProt: P15498 Human
- Unigene: 116237 Human
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Alternative names
- Oncogene vav antibody
- p95Vav antibody
- Proto-oncogene vav antibody
see all
Images
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Western blot - Anti-VAV1 antibody (ab245440)All lanes : Anti-VAV1 antibody (ab245440) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 15 µg per lane.
Exposure time: 30 secondsPrepared using NETN lysis buffer.
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Immunoprecipitation - Anti-VAV1 antibody (ab245440)VAV1 was immunoprecipitated from Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab245440 used for IP at 20 µl per reaction. For WB at a 1/250 dilution.Lane 1: ab245440 IP in Jurkat whole cell lysate.
Lane 2: Control IgG in Jurkat whole cell lysate.
Chemiluminescence detection: 30 seconds.
Datasheets and documents
References
ab245440 has not yet been referenced specifically in any publications.
