Recombinant Anti-VAV1 (phospho Y174) antibody [EP510Y] (Alexa Fluor® 488) (ab237470)


  • Product name

    Anti-VAV1 (phospho Y174) antibody [EP510Y] (Alexa Fluor® 488)
    See all VAV1 primary antibodies
  • Description

    Rabbit monoclonal [EP510Y] to VAV1 (phospho Y174) (Alexa Fluor® 488)
  • Host species

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human VAV1 (phospho Y174). The exact sequence is proprietary.

  • Positive control

    • Flow Cyt: Jurkat cells treated with Pervandate (1mM, 30 min). ICC/IF: Jurkat cells treated with Pervandate (1mM, 30 min)
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab237470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500.
ICC/IF 1/200.

This product gave a positive signal in Jurkat cells treated with Pervanadate (1mM, 30min) fixed with 4% formaldehyde (10 min)


  • Function

    Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.
  • Tissue specificity

    Widely expressed in hematopoietic cells but not in other cell types.
  • Sequence similarities

    Contains 1 CH (calponin-homology) domain.
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 SH2 domain.
    Contains 2 SH3 domains.
  • Domain

    The DH domain is involved in interaction with CCPG1.
  • Post-translational

    Phosphorylated on tyrosine residues.
  • Information by UniProt
  • Database links

  • Alternative names

    • Oncogene vav antibody
    • p95Vav antibody
    • Proto-oncogene vav antibody
    • Protooncogene vav antibody
    • VAV 1 antibody
    • VAV 1 oncogene antibody
    • VAV antibody
    • Vav proto oncogene antibody
    • VAV_HUMAN antibody
    • VAV1 antibody
    see all


  • ab237470 staining VAV1 (phospho Y174) in Jurkat cells +/- Pervanadate (1mM, 30min). The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h.

    The cells were then incubated overnight at +4°C with ab237470 at 1/200 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Overlay histogram showing Jurkat cells untreated (green line) and Jurkat cells treated with Pervanadate, 1mM, 30 minutes, (red line) stained with ab237470. The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab237470, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20mW solid-state laser (488nm) and 530/30 bandpass filter.


ab237470 has not yet been referenced specifically in any publications.

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