Recombinant
RabMAb

Recombinant Anti-VAV1 (phospho Y174) antibody [EP510Y] - BSA and Azide free (ab238424)

Overview

  • Product name

    Anti-VAV1 (phospho Y174) antibody [EP510Y] - BSA and Azide free
    See all VAV1 primary antibodies
  • Description

    Rabbit monoclonal [EP510Y] to VAV1 (phospho Y174) - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    This antibody detects VAV1 phosphorylated at Tyrosine 174.
  • Tested applications

    Suitable for: WB, Flow Cyt, Dot blot, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human VAV1 (phospho Y174). The exact sequence is proprietary.

  • Positive control

    • ICC/IF: Jurkat cells treated with Pervanadate (1 mM, 30 minutes).
  • General notes

    ab238424 is the carrier-free version of ab76225 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab238424 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab238424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).
Flow Cyt Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.
    • Tissue specificity

      Widely expressed in hematopoietic cells but not in other cell types.
    • Sequence similarities

      Contains 1 CH (calponin-homology) domain.
      Contains 1 DH (DBL-homology) domain.
      Contains 1 PH domain.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 SH2 domain.
      Contains 2 SH3 domains.
    • Domain

      The DH domain is involved in interaction with CCPG1.
    • Post-translational
      modifications

      Phosphorylated on tyrosine residues.
    • Information by UniProt
    • Database links

    • Alternative names

      • Oncogene vav antibody
      • p95Vav antibody
      • Proto-oncogene vav antibody
      • Protooncogene vav antibody
      • VAV 1 antibody
      • VAV 1 oncogene antibody
      • VAV antibody
      • Vav proto oncogene antibody
      • VAV_HUMAN antibody
      • VAV1 antibody
      see all

    Images

    • Flow Cytometry analysis of Jurkat (human acute T cell leukemia) untreated/treated with 1mM pervanadate for 30min cells labeling VAV1 with unpurified ab76225 at 1/200 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control. Untreated control (Green).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76225).

    • Dot blot analysis of VAV1 (pY174) peptide (Lane 1) and VAV1 non-phospho peptide (Lane 2) labelling VAV1 (phospho Y174) with ab76225 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76225).

    • Immunocytochemistry/Immunofluorescence analysis of untreated Jurkat cells and Pervanadate (1 mM, 30 minutes) treated Jurkat cells labeling VAV1 (phospho Y174) with purified ab76225 at 1/500 dilution.

      Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100, and counterstained with ab7291 anti-Tubulin (mouse mAb) 1:1000 (1 µg/ml). An Alexa-Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (ab150077). Nuclei counterstained with DAPI (blue).

      Negative Control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120).

      Negative Control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077).

      This data was developed using the same antibody clone in a different buffer formulation containing Tris glycine, tissue culture supernatant, BSA, glycerol and sodium azide (ab76225).

    References

    ab238424 has not yet been referenced specifically in any publications.

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