Key features and details
- Rabbit polyclonal to VAV2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rabbit, Human
- Isotype: IgG
Product nameAnti-VAV2 antibody
See all VAV2 primary antibodies
DescriptionRabbit polyclonal to VAV2
Specificityab86699 detects VAV2 in human A431 cells and detects several VAV isoforms in Jurkat cells. The immunogen sequence has significant homology to the conserved site in VAV1 and VAV3.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rabbit, Human
Predicted to work with: Rat, Chicken, Orangutan
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab86699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).|
FunctionGuanine nucleotide exchange factor for the Rho family of Ras-related GTPases. Plays an important role in angiogenesis. Its recruitement by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly.
Tissue specificityWidely expressed.
Sequence similaritiesContains 1 CH (calponin-homology) domain.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 SH2 domain.
Contains 2 SH3 domains.
- Information by UniProt
- Guanine nucleotide exchange factor VAV2 antibody
- Oncogene VAV2 antibody
- Protein vav 2 antibody
All lanes : Anti-VAV2 antibody (ab86699) at 1/1000 dilution
Lane 1 : A431 cells treated with EGF
Lane 2 : A431 cells treated with EGF, exposed to alkaline
Predicted band size: 100 kDa
Observed band size: 100 kDa
Additional bands at: <100 kDa. We are unsure as to the identity of these extra bands.
Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
Immunocytochemical labeling of VAV2 in paraformaldehyde-fixed and NP40 permeabilized rabbit spleen fibroblasts. The cells were labeled with ab86699 at a 1/50 dilution and detected using an appropriate secondary antibody conjugated to Cy3.
ab86699 has been referenced in 1 publication.
- Bastounis EE et al. Subendothelial stiffness alters endothelial cell traction force generation while exerting a minimal effect on the transcriptome. Sci Rep 9:18209 (2019). PubMed: 31796790