Overview

  • Product name

    Anti-VAV2 antibody - C-terminal
    See all VAV2 primary antibodies
  • Description

    Rabbit polyclonal to VAV2 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human VAV2 (C terminal). The exact sequence is proprietary.
    Database link: P52735

  • Positive control

    • IP: HepG2 whole cell extract. WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts; Mouse olfactory bulb tissue extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab226955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 101 kDa.
IP 1/100 - 1/705.

Target

  • Function

    Guanine nucleotide exchange factor for the Rho family of Ras-related GTPases. Plays an important role in angiogenesis. Its recruitement by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Contains 1 CH (calponin-homology) domain.
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 SH2 domain.
    Contains 2 SH3 domains.
  • Information by UniProt
  • Database links

  • Alternative names

    • Guanine nucleotide exchange factor VAV2 antibody
    • Oncogene VAV2 antibody
    • Protein vav 2 antibody
    • VAV 2 antibody
    • Vav 2 oncogene antibody
    • VAV-2 antibody
    • VAV2 antibody
    • VAV2 oncogene antibody
    • VAV2_HUMAN antibody
    see all

Images

  • VAV2 was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract using 5 µg of ab226955. Western blot was performed from the immunoprecipitate using ab226955.

    Lane 1: Control IgG IP in HepG2 whole cell extract.

    Lane 2: ab226955 IP in HepG2 whole cell extract.

  • All lanes : Anti-VAV2 antibody - C-terminal (ab226955) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 101 kDa



    7.5% SDS-PAGE

  • Anti-VAV2 antibody - C-terminal (ab226955) at 1/3000 dilution + Mouse olfactory bulb tissue extract at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 101 kDa



    7.5% SDS-PAGE gel.

References

ab226955 has not yet been referenced specifically in any publications.

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