• Product name
    Anti-VAV2 antibody [EP1067Y]
    See all VAV2 primary antibodies
  • Description
    Rabbit monoclonal [EP1067Y] to VAV2
  • Host species
  • Specificity
    This antibody detects Vav2 phosphorylated on Tyr172 as well as unphosphorylated Vav2.
  • Tested applications
    Suitable for: WB, IP, ICC, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human VAV2. The exact sequence is proprietary.

  • Positive control
    • IHC-P: Human cervical carcinoma. WB: 293 cell lysate. Flow Cyt: HeLa cells. IP: HEK293 cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab52640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/20000. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).
IP Use at an assay dependent concentration.
ICC 1/100 - 1/250.
IHC-P Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Guanine nucleotide exchange factor for the Rho family of Ras-related GTPases. Plays an important role in angiogenesis. Its recruitement by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly.
    • Tissue specificity
      Widely expressed.
    • Sequence similarities
      Contains 1 CH (calponin-homology) domain.
      Contains 1 DH (DBL-homology) domain.
      Contains 1 PH domain.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 SH2 domain.
      Contains 2 SH3 domains.
    • Information by UniProt
    • Database links
    • Alternative names
      • Guanine nucleotide exchange factor VAV2 antibody
      • Oncogene VAV2 antibody
      • Protein vav 2 antibody
      • VAV 2 antibody
      • Vav 2 oncogene antibody
      • VAV-2 antibody
      • VAV2 antibody
      • VAV2 oncogene antibody
      • VAV2_HUMAN antibody
      see all


    • All lanes : Anti-VAV2 antibody [EP1067Y] (ab52640) at 1/20000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : VAV2 knockout HAP1 whole cell lysate
      Lane 3 : HeLa whole cell lysate
      Lane 4 : MCF7 whole cell lysate

      Lysates/proteins at 1/20 dilution per lane.

      Predicted band size: 101 kDa

      Lanes 1 - 4: Merged signal (red and green). Green - ab52640 observed at 101 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab52640 was shown to specifically react with VAV2 in wild-type HAP1 cells as signal was lost in VAV2 knockout cells. Wild-type and VAV2 knockout samples were subjected to SDS-PAGE. Ab52640 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/20000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Human cervical carcinoma stained with ab52640 at 1/50 - 1/100 dilution
    • Anti-VAV2 antibody [EP1067Y] (ab52640) at 1/20000 dilution + 293 cell lysate at 10 µg

      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size: 101 kDa
      Observed band size: 101 kDa

    • Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling VAV2 (red) with ab52640 at a 1/2500 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

    • VAV2 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 10µg of Rabbit monoclonal to VAV2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
      The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
      Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab52640.
      Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
      Band: 100kDa; VAV2.


    This product has been referenced in:
    • Lehman HL  et al. NFkB hyperactivation causes invasion of esophageal squamous cell carcinoma with EGFR overexpression and p120-catenin down-regulation. Oncotarget 9:11180-11196 (2018). Read more (PubMed: 29541406) »
    • Hu J  et al. Ubiquitin E3 Ligase MARCH7 promotes proliferation and invasion of cervical cancer cells through VAV2-RAC1-CDC42 pathway. Oncol Lett 16:2312-2318 (2018). Read more (PubMed: 30008934) »
    See all 15 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Human Cell lysate - whole cell (HEK293)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

    Abcam user community

    Verified customer

    Submitted Dec 03 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Western blot
    Human Cell lysate - whole cell (human and mouse cell lines)
    Loading amount
    50 µg
    human and mouse cell lines
    Gel Running Conditions
    Reduced Denaturing (8)
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Jul 28 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Western blot
    Human Cell lysate - whole cell (HT29 cells)
    Loading amount
    20 µg
    HT29 cells
    EPA for 24 hrs
    Gel Running Conditions
    Reduced Denaturing (10% bis tris gel)
    Blocking step
    Thermo scientific superblock buffer in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C

    Dr. Joanne Doleman

    Verified customer

    Submitted Oct 22 2008


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