Recombinant Anti-VAV2 antibody [EP1067Y] - BSA and Azide free (ab239846)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-VAV2 antibody [EP1067Y] - BSA and Azide free
See all VAV2 primary antibodies -
Description
Rabbit monoclonal [EP1067Y] to VAV2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, ICC, IHC-P, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human VAV2. The exact sequence is proprietary.
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General notes
Ab239846 is the carrier-free version of ab52640. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab239846 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1067Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab239846 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| ICC | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| Flow Cyt | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa). |
Target
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Function
Guanine nucleotide exchange factor for the Rho family of Ras-related GTPases. Plays an important role in angiogenesis. Its recruitement by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. -
Tissue specificity
Widely expressed. -
Sequence similarities
Contains 1 CH (calponin-homology) domain.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 SH2 domain.
Contains 2 SH3 domains. - Information by UniProt
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Database links
- Entrez Gene: 7410 Human
- Entrez Gene: 22325 Mouse
- Entrez Gene: 296603 Rat
- Omim: 600428 Human
- SwissProt: P52735 Human
- SwissProt: Q60992 Mouse
- Unigene: 369921 Human
- Unigene: 689325 Human
see all -
Alternative names
- Guanine nucleotide exchange factor VAV2 antibody
- Oncogene VAV2 antibody
- Protein vav 2 antibody
see all
Images
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![Flow Cytometry - Anti-VAV2 antibody [EP1067Y] - BSA and Azide free (ab239846)](https://www.abcam.com/ps/products/239/ab239846/Images/ab239846-3-ab526404ab52640FC1.jpg)
Flow Cytometry - Anti-VAV2 antibody [EP1067Y] - BSA and Azide free (ab239846)Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling VAV2 (red) with ab52640 at a 1/2500 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52640).
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![Immunoprecipitation - Anti-VAV2 antibody [EP1067Y] - BSA and Azide free (ab239846)](https://www.abcam.com/ps/products/239/ab239846/Images/ab239846-2-VAV2Primaryantibodiesab526403.jpg)
Immunoprecipitation - Anti-VAV2 antibody [EP1067Y] - BSA and Azide free (ab239846)VAV2 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 10µg of Rabbit monoclonal to VAV2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab52640.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 100kDa; VAV2.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52640).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAV2 antibody [EP1067Y] - BSA and Azide free (ab239846)](https://www.abcam.com/ps/products/239/ab239846/Images/ab239846-1-vav2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAV2 antibody [EP1067Y] - BSA and Azide free (ab239846)Human cervical carcinoma stained with ab52640 at 1/50 - 1/100 dilution
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52640).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Datasheets and documents
References
ab239846 has not yet been referenced specifically in any publications.
