Product nameAnti-VAV3 antibody [EP1130Y]
See all VAV3 primary antibodies
DescriptionRabbit monoclonal [EP1130Y] to VAV3
Tested applicationsSuitable for: WB, IP, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human VAV3 (C terminal). The exact sequence is proprietary.
- Jurkat whole cell lysate (ab7899); permeabalized Jurkat cells.
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab52938 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use at an assay dependent concentration. PubMed: 23566222
Methanol fixed cells
FunctionExchange factor for GTP-binding proteins RhoA, RhoG and, to a lesser extent, Rac1. Binds physically to the nucleotide-free states of those GTPases. Plays an important role in angiogenesis. Its recruitement by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly.
Sequence similaritiesContains 1 CH (calponin-homology) domain.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 SH2 domain.
Contains 2 SH3 domains.
- Information by UniProt
- FLJ40431 antibody
- Guanine nucleotide exchange factor VAV3 antibody
- Protein vav 3 antibody
Anti-VAV3 antibody [EP1130Y] (ab52938) at 1/100000 dilution + Jurkat cell lysate at 10 µg
HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 98 kDa
Observed band size: ~98 kDa why is the actual band size different from the predicted?
Flow cytometric analysis of permeabilized Jurkat cells using ab52938 (red) or a rabbit IgG (negative) (green).
This product has been referenced in:
- Maruyama K et al. Bone-protective Functions of Netrin 1 Protein. J Biol Chem 291:23854-23868 (2016). Read more (PubMed: 27681594) »
- Aguilar H et al. VAV3 mediates resistance to breast cancer endocrine therapy. Breast Cancer Res 16:R53 (2014). Mouse . Read more (PubMed: 24886537) »