Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1130Y] to VAV3 - BSA and Azide free
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Human
Product nameAnti-VAV3 antibody [EP1130Y] - BSA and Azide free
See all VAV3 primary antibodies
DescriptionRabbit monoclonal [EP1130Y] to VAV3 - BSA and Azide free
Tested applicationsSuitable for: Flow Cyt, WBmore details
Unsuitable for: ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human VAV3 (C terminal). The exact sequence is proprietary.
ab247340 is the carrier-free version of ab52938 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247340 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab247340 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).|
FunctionExchange factor for GTP-binding proteins RhoA, RhoG and, to a lesser extent, Rac1. Binds physically to the nucleotide-free states of those GTPases. Plays an important role in angiogenesis. Its recruitement by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly.
Sequence similaritiesContains 1 CH (calponin-homology) domain.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 SH2 domain.
Contains 2 SH3 domains.
- Information by UniProt
- FLJ40431 antibody
- Guanine nucleotide exchange factor VAV3 antibody
- Protein vav 3 antibody
Anti-VAV3 antibody [EP1130Y] (ab52938) at 1/100000 dilution + Jurkat cell lysate at 10 µg
HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 98 kDa
Observed band size: ~98 kDa why is the actual band size different from the predicted?
This data was developed using ab52938, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab247340 has not yet been referenced specifically in any publications.