Overview

  • Product name

    Anti-VAV3 (phospho Y173) antibody [EPR1068A]
    See all VAV3 primary antibodies
  • Description

    Rabbit monoclonal [EPR1068A] to VAV3 (phospho Y173)
  • Host species

    Rabbit
  • Specificity

    This antibody only detects VAV3 phosphorylated at Tyrosine 173.
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding tyrosine 173 of Human VAV3

  • Positive control

    • Jurkat cell lysates
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 98 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Exchange factor for GTP-binding proteins RhoA, RhoG and, to a lesser extent, Rac1. Binds physically to the nucleotide-free states of those GTPases. Plays an important role in angiogenesis. Its recruitement by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly.
    • Sequence similarities

      Contains 1 CH (calponin-homology) domain.
      Contains 1 DH (DBL-homology) domain.
      Contains 1 PH domain.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 SH2 domain.
      Contains 2 SH3 domains.
    • Information by UniProt
    • Database links

    • Alternative names

      • FLJ40431 antibody
      • Guanine nucleotide exchange factor VAV3 antibody
      • Protein vav 3 antibody
      • Protein vav3 antibody
      • RGD1565941 antibody
      • VAV 3 antibody
      • Vav 3 guanine nucleotide exchange factor antibody
      • VAV 3 oncogene antibody
      • VAV 3 protein antibody
      • VAV-3 antibody
      • Vav3 antibody
      • VAV3 oncogene antibody
      • VAV3 protein antibody
      • VAV3_HUMAN antibody
      see all

    Images

    • All lanes : Anti-VAV3 (phospho Y173) antibody [EPR1068A] (ab109544) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Jurkat cell lysate treated with Pervanadate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 98 kDa

    References

    This product has been referenced in:

    • Maruyama K  et al. Bone-protective Functions of Netrin 1 Protein. J Biol Chem 291:23854-23868 (2016). Read more (PubMed: 27681594) »
    • Kovárová D  et al. Downregulation of HOPX controls metastatic behavior in sarcoma cells and identifies genes associated with metastasis. Mol Cancer Res 11:1235-47 (2013). Read more (PubMed: 23938949) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Answer

    Thank you for contacting us.


    Unfortunately, we do not know of any publications that have used this antibody at the moment. But I would like to reassure you that ab109544 is tested and guaranteed to work in WB with human samples.

    Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

    We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

    If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

    Read More

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