Product nameAnti-VAX2 antibody [EPR10424]
See all VAX2 primary antibodies
DescriptionRabbit monoclonal [EPR10424] to VAX2
SpecificityThe alignment of the immunogen sequence of ab154855 and human and rat VAX1 shows a high homology of 92% (human) and 93% (rat). Based on the sequence, a cross-reaction of ab154855 with VAX1 is very likely, although this has not been experimentally tested by us.
Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Rat, Human
Synthetic peptide, corresponding to residues in Human VAX2 (Uniprot ID Q9UIW0).
- Jurkat, HeLa, Y79 cell lysates; Y79 cells.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab154855 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 31 kDa.|
|ICC/IF||1/100 - 1/250.|
RelevanceVAX2 belongs to the EMX homeobox family and contains 1 homeobox DNA-binding domain. VAX2 gene has high sequence similarity to VAX1 gene and is almost exclusively expressed in the ventral portion of the retina during development. It may play an important role in morphogenetic processes underlying the correct development of the eye, particularly in the establishment of a correct dorsoventral pattern and may be a regulator of axial polarization in the retina.
- DRES93 antibody
- VAX2 antibody
- Ventral anterior homeobox 2 antibody
All lanes : Anti-VAX2 antibody [EPR10424] (ab154855) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Y79 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 31 kDa
Immunofluorescent analysis of Y79 cells labeling VAX2 with ab154855 at 1/100 dilution.
ab154855 has not yet been referenced specifically in any publications.