Overview

  • Product name

    Anti-VCAM1 antibody [324]
    See all VCAM1 primary antibodies
  • Description

    Rat monoclonal [324] to VCAM1
  • Host species

    Rat
  • Tested applications

    Suitable for: WB, ICC, IHC (Methanol fixed), IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Glycoprotein fraction from cerebellum of 8-10 day old C57BL/6J mice.

  • Positive control

    • Brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab78712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 20 µg/ml. Predicted molecular weight: 81 kDa.
ICC Use a concentration of 5 - 10 µg/ml.
IHC (Methanol fixed) Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution. Fresh frozen, acetone or -20C methanol fixed tissues are recommended. Traditional 4% PFA typically does not work well. Other fixatives have not been tested.

Target

  • Function

    Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
  • Tissue specificity

    Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
  • Sequence similarities

    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
  • Domain

    Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
  • Post-translational
    modifications

    Sialoglycoprotein.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD106 antibody
    • CD106 Antigen antibody
    • INCAM 100 antibody
    • INCAM-100 antibody
    • L1CAM antibody
    • MGC99561 antibody
    • V-CAM 1 antibody
    • Vascular Cell Adhesion Molecule 1 antibody
    • Vascular cell adhesion protein 1 antibody
    • VCAM 1 antibody
    • VCAM-1 antibody
    • VCAM1 antibody
    • VCAM1_HUMAN antibody
    see all

References

This product has been referenced in:

  • Dai C  et al. Apolipoprotein A-I attenuates ovalbumin-induced neutrophilic airway inflammation via a granulocyte colony-stimulating factor-dependent mechanism. Am J Respir Cell Mol Biol 47:186-95 (2012). WB ; Mouse . Read more (PubMed: 22427535) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse brain)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
Mouse brain
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Nov 05 2018

Answer

Thank you very much for your interest in ab our VCAM1 antibodies.


I have checked our IHC-P-guaranteed VCAM1 antibodies for their potential cross-reactivity with rabbit tissue, and based on the immunogen sequence similarity of 86% I may indeed recommend the mouse monoclonal ab98954.

To our knowledge, ab98954 has not been tested in rabbit but may work based on the sequence similarity. Therefore, I can offer a discount off a future purchase if you buy ab98954 now, test it in rabbit in IHC-P and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.





If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab98954. I will then send a discount code. This code must be issued before purchasing the antibody so please wait for my reply before ordering.





2. Purchase the antibody either by phone, fax, or online (www.abcam.com).

3. Test it in rabbit.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab98954 turns out to be unsuitable for rabbit, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount

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Answer

Perdona por la tardanza en contestar, estaba chequeando con mis compañeros del laboratorio las distintas opciones de reactividad cruzada de este anticuerpo con conejo. Desgraciadamente no hay muchos datos al respecto, ya que ninguno de ellos se ha testado en esta especie, y muchos de los epítopos no han sido mapeados, por lo que no puede llevarse a cabo un BLAST.

Así pues, si sigues interesada en testarlo en conejo, a pesar de no tener datos concretos acerca de las posibilidades de reactividad, te puedo mandar un código para canjear por un anticuerpo gratuito si nos envías los resultados (independientemente de si estos resultan positivos o no).

Para detectar I CAM1 yo me inclinaría por ab25375 o ab27294, y en el caso de V CAM1 por ab78712 o ab98954.

Es importante que antes de realizar el pedido te emita el código valido para el anticuerpo gratuito por participar en el sistema Abreviews, así que te pediría que antes de hacer el pedido, por favor, esperes a que te lo comunique.

¿Qué tal han ido los resultados con ab25072?

Respecto a la duda de los Abreviews, te cuento. Si se trata de una aplicación o especie que no se ha testado hasta el momento, la compensación por participar en el sistema Abreview es un código (emitido ANTES de realizar el pedido) válido por un anticuerpo primario completamente gratuito, y también una serie de puntos (Abpoints) canjeables por distintas opciones.

Si, por el contrario, los resultados enviados corresponden a información adicional sobre especies o aplicaciones ya testadas, en ese caso se ofrecen (Abpoints), pues ayudan a completar la información y referencias del anticuerpo.

Respecto a los anticuerpos que ya habéis probado en el pasado, siempre podéis publicar los resultados, pero aunque sean nuevas aplicaciones / especies no podremos ofreceros un código de descuento ya que el pedido se ha realizado con anterioridad a dicho código. Aun así, podéis mandar los resultados, y ser compensados con puntos.

Espero haberte aclarado. Quedo a la espera de recibir tu respuesta para que me indiques como proceder. Si tienes más dudas, por favor, no dudes en volvernos a contactar.

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