Anti-VCAM1 antibody [B-K9] (ab47159)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-VCAM1 antibody [B-K9]
See all VCAM1 primary antibodies -
DescriptionMouse monoclonal [B-K9] to VCAM1
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Host speciesMouse
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SpecificityRecognises the soluble antigen
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Tested applicationsSuitable for: Blocking, Functional Studies, Flow Cytmore details
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Species reactivityReacts with: Human
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Immunogen
Tissue/ cell preparation of activated human umbilical vein endothelial cells (HUVEC).
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferConstituent: PBS
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Concentration information loading... -
PurityIon Exchange Chromatography
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Purification notesSterile-filtered through 0.22 µm and treated to remove endotoxins. Carrier and preservative free
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ClonalityMonoclonal
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Clone numberB-K9
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Myelomax63-Ag8.653
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab47159 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Blocking | Use at an assay dependent concentration. Blocks the cell attachment to VCAM1. | |
| Functional Studies | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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FunctionImportant in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
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Tissue specificityExpressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
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Sequence similaritiesContains 7 Ig-like C2-type (immunoglobulin-like) domains.
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DomainEither the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
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Post-translational
modificationsSialoglycoprotein. -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 7412 Human
- GenBank: NP_001069.1 Human
- GenBank: NP_001186763.1 Human
- Omim: 192225 Human
- SwissProt: P19320 Human
- Unigene: 109225 Human
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Alternative names
- CD106 antibody
- CD106 Antigen antibody
- INCAM 100 antibody
see all
Images
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![Flow Cytometry - Anti-VCAM1 antibody [B-K9] (ab47159)](https://www.abcam.com/ps/products/47/ab47159/Images/47159.gif)
Flow Cytometry - Anti-VCAM1 antibody [B-K9] (ab47159)A typical staining pattern with the B-K9 monoclonal antibody of VCAM-1 transfected CHO cell line
Protocols
Datasheets and documents
References
This product has been referenced in:
- Lefèvre S et al. Synovial fibroblasts spread rheumatoid arthritis to unaffected joints. Nat Med 15:1414-20 (2009). Functional Studies ; Human . Read more (PubMed: 19898488) »
