Anti-VCAM1 antibody [B-K9] (ab47159)

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Overview

  • Product name

    Anti-VCAM1 antibody [B-K9]
    See all VCAM1 primary antibodies

  • Description

    Mouse monoclonal [B-K9] to VCAM1

  • Host species

    Mouse

  • Specificity

    Recognises the soluble antigen

  • Tested applications

    Suitable for: Blocking, Functional Studies, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Tissue/ cell preparation of activated human umbilical vein endothelial cells (HUVEC).

Properties

Applications

Our Abpromise guarantee covers the use of ab47159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Blocking Use at an assay dependent concentration. Blocks the cell attachment to VCAM1.
Functional Studies Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.

  • Tissue specificity

    Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.

  • Sequence similarities

    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.

  • Domain

    Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.

  • Post-translational
    modifications

    Sialoglycoprotein.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD106 antibody
      • CD106 Antigen antibody
      • INCAM 100 antibody
      • INCAM-100 antibody
      • L1CAM antibody
      • MGC99561 antibody
      • V-CAM 1 antibody
      • Vascular Cell Adhesion Molecule 1 antibody
      • Vascular cell adhesion protein 1 antibody
      • VCAM 1 antibody
      • VCAM-1 antibody
      • VCAM1 antibody
      • VCAM1_HUMAN antibody
      see all

    Images

    • Flow Cytometry - Anti-VCAM1 antibody [B-K9] (ab47159)
      Flow Cytometry - Anti-VCAM1 antibody [B-K9] (ab47159)
      A typical staining pattern with the B-K9 monoclonal antibody of VCAM-1 transfected CHO cell line

    References

    This product has been referenced in:

    • Pichavaram P  et al. Cholesterol crystals promote endothelial cell and monocyte interactions via H2O2-mediated PP2A inhibition, NF?B activation and ICAM1 and VCAM1 expression. Redox Biol 24:101180 (2019). Read more (PubMed: 31022672) »
    • Lefèvre S  et al. Synovial fibroblasts spread rheumatoid arthritis to unaffected joints. Nat Med 15:1414-20 (2009). Functional Studies ; Human . Read more (PubMed: 19898488) »
    See all 2 Publications for this product

    Publishing research using ab47159? Please let us know so that we can cite the reference in this datasheet.

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