Recombinant
RabMAb

Recombinant Anti-VCAM1 antibody [EPR5038(2)] (ab174279)

Rabbit recombinant monoclonal VCAM1 antibody [EPR5038(2)]. Validated in WB, IP and tested in Mouse, Rat, Human. Cited in 5 publication(s).

Overview

  • Product name

    Anti-VCAM1 antibody [EPR5038(2)]
    See all VCAM1 primary antibodies
  • Description

    Rabbit monoclonal [EPR5038(2)] to VCAM1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human VCAM1 aa 700 to the C-terminus. The exact sequence is proprietary.
    Database link: P19320

  • Positive control

    • Human fetal kidney, HuT-78 and NIH3T3 lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab174279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 81 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
    • Tissue specificity

      Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
    • Sequence similarities

      Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    • Domain

      Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
    • Post-translational
      modifications

      Sialoglycoprotein.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD106 antibody
      • CD106 Antigen antibody
      • INCAM 100 antibody
      • INCAM-100 antibody
      • L1CAM antibody
      • MGC99561 antibody
      • V-CAM 1 antibody
      • Vascular Cell Adhesion Molecule 1 antibody
      • Vascular cell adhesion protein 1 antibody
      • VCAM 1 antibody
      • VCAM-1 antibody
      • VCAM1 antibody
      • VCAM1_HUMAN antibody
      see all

    Images

    • All lanes : Anti-VCAM1 antibody [EPR5038(2)] (ab174279) at 1/1000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : HuT-78 lysate
      Lane 3 : NIH3T3 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 81 kDa

    • Immunoprecipitation. ab174279 at 1/1000 labeling VCAM1 immunoprecipitated from NIH3T3 cell lysate using ab174279 at 1/10.

    References

    This product has been referenced in:

    • Xu L  et al. Peroxisome Proliferator-Activated Receptor-? Antagonizes LOX-1-Mediated Endothelial Injury by Transcriptional Activation of miR-590-5p. PPAR Res 2019:2715176 (2019). Read more (PubMed: 31354796) »
    • Belcher JD  et al. Oral carbon monoxide therapy in murine sickle cell disease: Beneficial effects on vaso-occlusion, inflammation and anemia. PLoS One 13:e0205194 (2018). Read more (PubMed: 30308028) »
    See all 7 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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