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  1. Link

    vcam1-antibody-epr50382-bsa-and-azide-free-ab249790.pdf

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Cell Adhesion Molecules Vascular
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Recombinant
RabMAb

Recombinant Anti-VCAM1 antibody [EPR5038(2)] - BSA and Azide free (ab249790)

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  • Datasheet
  • References
  • Protocols

Overview

  • Product name

    Anti-VCAM1 antibody [EPR5038(2)] - BSA and Azide free
    See all VCAM1 primary antibodies
  • Description

    Rabbit monoclonal [EPR5038(2)] to VCAM1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human VCAM1 aa 700 to the C-terminus. The exact sequence is proprietary.
    Database link: P19320

  • General notes

    Ab249790 is the carrier-free version of ab174279. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab249790 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5038(2)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cell Adhesion Molecules
    • Vascular
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cell Adhesion Molecules
    • Endothelial
    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Stem Cells
    • Mesenchymal Stem Cells
    • Surface Molecules
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Other
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Cell adhesion molecules
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Adhesion molecules ELISA kits
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Atherosclerotic proteins ELISA kits
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

Associated products

  • Alternative Versions

    • Anti-VCAM1 antibody [EPR5038(2)] (ab174279)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit (ab102918)
    • APC Conjugation Kit (ab201807)
  • Positive Controls

    • NIH 3T3 whole cell lysate (ab7179)

Applications

Our Abpromise guarantee covers the use of ab249790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 100 kDa (predicted molecular weight: 81 kDa).
IP Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
    • Tissue specificity

      Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
    • Sequence similarities

      Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    • Domain

      Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
    • Post-translational
      modifications

      Sialoglycoprotein.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P19320 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 7412 Human
      • Entrez Gene: 22329 Mouse
      • Entrez Gene: 25361 Rat
      • GenBank: NP_001069.1 Human
      • GenBank: NP_001186763.1 Human
      • Omim: 192225 Human
      • SwissProt: P19320 Human
      • SwissProt: P29533 Mouse
      • SwissProt: P29534 Rat
      • Unigene: 109225 Human
      • Unigene: 440909 Mouse
      • Unigene: 76649 Mouse
      • Unigene: 11267 Rat
      see all
    • Alternative names

      • CD106 antibody
      • CD106 Antigen antibody
      • INCAM 100 antibody
      • INCAM-100 antibody
      • L1CAM antibody
      • MGC99561 antibody
      • V-CAM 1 antibody
      • Vascular Cell Adhesion Molecule 1 antibody
      • Vascular cell adhesion protein 1 antibody
      • VCAM 1 antibody
      • VCAM-1 antibody
      • VCAM1 antibody
      • VCAM1_HUMAN antibody
      see all

    Datasheets and documents

    • Datasheet
  • References

    ab249790 has not yet been referenced specifically in any publications.

    Publishing research using ab249790? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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