RecombinantRabMAb

Recombinant Anti-VCAM1 antibody [EPR5038(2)] - BSA and Azide free (ab249790)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5038(2)] to VCAM1 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-VCAM1 antibody [EPR5038(2)] - BSA and Azide free
    See all VCAM1 primary antibodies

  • Description

    Rabbit monoclonal [EPR5038(2)] to VCAM1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human VCAM1 aa 700 to the C-terminus. The exact sequence is proprietary.
    Database link: P19320

  • General notes

    Ab249790 is the carrier-free version of ab174279. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab249790 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Applications

Our Abpromise guarantee covers the use of ab249790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 100 kDa (predicted molecular weight: 81 kDa).
IP Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

    • Target

    • Function

      Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.

    • Tissue specificity

      Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.

    • Sequence similarities

      Contains 7 Ig-like C2-type (immunoglobulin-like) domains.

    • Domain

      Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.

    • Post-translational
      modifications

      Sialoglycoprotein.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • CD106 antibody
      • CD106 Antigen antibody
      • INCAM 100 antibody
      • INCAM-100 antibody
      • L1CAM antibody
      • MGC99561 antibody
      • V-CAM 1 antibody
      • Vascular Cell Adhesion Molecule 1 antibody
      • Vascular cell adhesion protein 1 antibody
      • VCAM 1 antibody
      • VCAM-1 antibody
      • VCAM1 antibody
      • VCAM1_HUMAN antibody
      see all

    Images

    • Western blot - Anti-VCAM1 antibody [EPR5038(2)] - BSA and Azide free (ab249790)
      Western blot - Anti-VCAM1 antibody [EPR5038(2)] - BSA and Azide free (ab249790)
      All lanes : Anti-VCAM1 antibody [EPR5038(2)] (ab174279) at 1/1000 dilution

      Lane 1 : HUVEC (Human umbilical vein endothelial cell) whole cell lysate
      Lane 2 : HUVEC treated with 10 ng/ml TNF-a for 16 h, whole cell lysate
      Lane 3 : bEND.3 (Mouse brain endothelioma) whole cell lysate
      Lane 4 : bEND.3 treated with 10 µg/ml LPS for 24 h, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 81 kDa
      Observed band size: 100 kDa
      why is the actual band size different from the predicted?



      This data was developed using ab174279, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-VCAM1 antibody [EPR5038(2)] - BSA and Azide free (ab249790)
      Western blot - Anti-VCAM1 antibody [EPR5038(2)] - BSA and Azide free (ab249790)
      All lanes : Anti-VCAM1 antibody [EPR5038(2)] (ab174279) at 1/1000 dilution

      Lane 1 : Hut-78 (Human Sezary syndrome cutaneous T lymphocyte) whole cell lysate
      Lane 2 : SK-OV-3 (Human ovarian cancer epithelial cell) whole cell lysate
      Lane 3 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
      Lane 4 : J774A.1 (Mouse reticulum cell sarcoma monocyte macrophage) whole cell lysate
      Lane 5 : LADMAC (Mouse bone marrow monocyte macrophage) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 81 kDa
      Observed band size: 100 kDa why is the actual band size different from the predicted?


      Exposure time: 15 seconds


      This data was developed using ab174279, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-VCAM1 antibody [EPR5038(2)] - BSA and Azide free (ab249790)
      Western blot - Anti-VCAM1 antibody [EPR5038(2)] - BSA and Azide free (ab249790)
      All lanes : Anti-VCAM1 antibody [EPR5038(2)] (ab174279) at 1/1000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : HuT-78 lysate
      Lane 3 : NIH3T3 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 81 kDa



      This data was developed using ab174279, the same antibody clone in a different buffer formulation.

    • Immunoprecipitation - Anti-VCAM1 antibody [EPR5038(2)] - BSA and Azide free (ab249790)
      Immunoprecipitation - Anti-VCAM1 antibody [EPR5038(2)] - BSA and Azide free (ab249790)

      This data was developed using ab174279, the same antibody clone in a different buffer formulation.

      Immunoprecipitation. ab174279 at 1/1000 labeling VCAM1 immunoprecipitated from NIH3T3 cell lysate using ab174279 at 1/10.

    • Anti-VCAM1 antibody [EPR5038(2)] - BSA and Azide free (ab249790)
      Anti-VCAM1 antibody [EPR5038(2)] - BSA and Azide free (ab249790)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab249790? Please let us know so that we can cite the reference in this datasheet.

    ab249790 has not yet been referenced specifically in any publications.

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