Product nameAnti-VCAM1 antibody [EPR5047] (Allophycocyanin)
See all VCAM1 primary antibodies
DescriptionRabbit monoclonal [EPR5047] to VCAM1 (Allophycocyanin)
ConjugationAllophycocyanin. Ex: 645nm, Em: 660nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Human
corresponding to Mouse VCAM1.
Database link: P19320
- Flow Cyt: NIH3T3 cells
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
Storage bufferpH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityProtein A purified
- Anti-VCAM1 antibody [EPR5047] (ab134047)
- Anti-VCAM1 antibody [EPR5047] (Alexa Fluor® 647) (ab194319)
- Anti-VCAM1 antibody [EPR5047] (HRP) (ab195540)
- Anti-VCAM1 antibody [EPR5047] - Low endotoxin, Azide free (ab215380)
- Anti-VCAM1 antibody [EPR5047] (Phycoerythrin) (ab223981)
- Anti-VCAM1 antibody [EPR5047] (FITC) (ab223982)
Our Abpromise guarantee covers the use of ab223983 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The cellular localisation of this product has been verified in ICC/IF.
FunctionImportant in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
Tissue specificityExpressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
Sequence similaritiesContains 7 Ig-like C2-type (immunoglobulin-like) domains.
DomainEither the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
- Information by UniProt
- CD106 antibody
- CD106 Antigen antibody
- INCAM 100 antibody
Overlay histogram showing NIH3T3 cells stained with ab223983 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223983, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Allophycocyanin (ab232814) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.
ab223983 has not yet been referenced specifically in any publications.