Product nameAnti-VCAM1 antibody [EPR5047] (HRP)
See all VCAM1 primary antibodies
DescriptionRabbit monoclonal [EPR5047] to VCAM1 (HRP)
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Mouse VCAM1. The exact sequence is proprietary.
(Peptide available as
- WB: Mouse Brain and Rat Brain tissue lysates. IHC-P: normal human tonsil tissue sections
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% Proclin
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Anti-VCAM1 antibody [EPR5047] (ab134047)
- Anti-VCAM1 antibody [EPR5047] (Alexa Fluor® 647) (ab194319)
- Anti-VCAM1 antibody [EPR5047] - Low endotoxin, Azide free (ab215380)
- Anti-VCAM1 antibody [EPR5047] (Phycoerythrin) (ab223981)
- Anti-VCAM1 antibody [EPR5047] (FITC) (ab223982)
- Anti-VCAM1 antibody [EPR5047] (Allophycocyanin) (ab223983)
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab195540 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ab199507 - Rabbit monoclonal IgG (HRP), is suitable for use an as isotype control with this antibody.
|WB||1/5000. Detects a band of approximately 95 kDa (predicted molecular weight: 81 kDa).Can be blocked with VCAM1 peptide (ab156177).|
FunctionImportant in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
Tissue specificityExpressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
Sequence similaritiesContains 7 Ig-like C2-type (immunoglobulin-like) domains.
DomainEither the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
- Information by UniProt
- CD106 antibody
- CD106 Antigen antibody
- INCAM 100 antibody
IHC image of VCAM1 staining in a section of formalin-fixed paraffin-embedded normal human tonsil*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with ab195540, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
All lanes : Anti-VCAM1 antibody [EPR5047] (HRP) (ab195540) at 1/5000 dilution
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 81 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab195540 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab195540 has not yet been referenced specifically in any publications.