FITC Anti-VCAM1 antibody [M/K-2] (ab24853)
Key features and details
- FITC Rat monoclonal [M/K-2] to VCAM1
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
FITC Anti-VCAM1 antibody [M/K-2]
See all VCAM1 primary antibodies -
Description
FITC Rat monoclonal [M/K-2] to VCAM1 -
Host species
Rat -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse VCAM1. BALB/3T3 and +/+2.4 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4 -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
M/K-2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 1µg for 106 cells.
ab18404 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt
Use 1µg for 106 cells. ab18404 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. -
Tissue specificity
Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue. -
Sequence similarities
Contains 7 Ig-like C2-type (immunoglobulin-like) domains. -
Domain
Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion. -
Post-translational
modificationsSialoglycoprotein. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 22329 Mouse
- SwissProt: P29533 Mouse
- Unigene: 440909 Mouse
- Unigene: 76649 Mouse
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Alternative names
- CD106 antibody
- CD106 Antigen antibody
- INCAM 100 antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (6)
ab24853 has been referenced in 6 publications.
- Gkouveris I et al. Vasculature submucosal changes at early stages of osteonecrosis of the jaw (ONJ). Bone 123:234-245 (2019). PubMed: 30953717
- Pagoto A et al. Paramagnetic Phospholipid-Based Micelles Targeting VCAM-1 Receptors for MRI Visualization of Inflammation. Bioconjug Chem 27:1921-30 (2016). PubMed: 27315634
- Zhang ZH et al. Protective effects of BMSCs in combination with erythropoietin in bronchopulmonary dysplasia-induced lung injury. Mol Med Rep 14:1302-8 (2016). PubMed: 27279073
- Zeng SL et al. Mesenchymal stem cells abrogate experimental asthma by altering dendritic cell function. Mol Med Rep 12:2511-20 (2015). PubMed: 25936350
- Acton SE et al. Dendritic cells control fibroblastic reticular network tension and lymph node expansion. Nature 514:498-502 (2014). Flow Cyt . PubMed: 25341788
- Ma M et al. Major histocompatibility complex-I expression on embryonic stem cell-derived vascular progenitor cells is critical for syngeneic transplant survival. Stem Cells 28:1465-75 (2010). Flow Cyt ; Mouse . PubMed: 20629173