PE Anti-VCAM1 antibody [M/K-2] (ab25452)
Key features and details
- PE Rat monoclonal [M/K-2] to VCAM1
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Overview
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Product name
PE Anti-VCAM1 antibody [M/K-2]
See all VCAM1 primary antibodies -
Description
PE Rat monoclonal [M/K-2] to VCAM1 -
Host species
Rat -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
ab25452 recognises CD106/VCAM1 (100 kDa) -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to VCAM1. BALB/3T3 and +/+2.4 cells
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Positive control
- Flow Cyt: Mouse bone marrow cells.
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General notes
The M/K-2 monoclonal antibody has been used in transplant studies to suppress cardiac rejection and induce long term cardiac graft survival. It immunoprecipitates a peptide that gives a single band on SDS-PAGE gels with an apparent Mr of ~100 kDa under reducing conditions and 92 kDa under nonreducing conditions.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.3
Preservative: 0.09% Sodium azide
Constituents: PBS, 16% Sucrose
Stabilising agent. -
Concentration information loading...
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Purity
Affinity purified -
Primary antibody notes
The M/K-2 monoclonal antibody has been used in transplant studies to suppress cardiac rejection and induce long term cardiac graft survival. It immunoprecipitates a peptide that gives a single band on SDS-PAGE gels with an apparent Mr of ~100 kDa under reducing conditions and 92 kDa under nonreducing conditions. -
Clonality
Monoclonal -
Clone number
M/K-2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25452 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 0.2µg for 106 cells.
ab154449 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt
Use 0.2µg for 106 cells. ab154449 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. -
Tissue specificity
Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue. -
Sequence similarities
Contains 7 Ig-like C2-type (immunoglobulin-like) domains. -
Domain
Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion. -
Post-translational
modificationsSialoglycoprotein. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 22329 Mouse
- SwissProt: P29533 Mouse
- Unigene: 440909 Mouse
- Unigene: 76649 Mouse
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Alternative names
- CD106 antibody
- CD106 Antigen antibody
- INCAM 100 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab25452 has been referenced in 1 publication.
- Olme CH et al. Live cell detection of chromosome 2 deletion and Sfpi1/PU1 loss in radiation-induced mouse acute myeloid leukaemia. Leuk Res 37:1374-82 (2013). Flow Cyt ; Mouse . PubMed: 23806234