Overview

  • Product name

    Anti-VCAM1 antibody - N-terminal
    See all VCAM1 primary antibodies
  • Description

    Rabbit polyclonal to VCAM1 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human VCAM1 (N terminal). The exact sequence is proprietary.
    Database link: P19320

  • Positive control

    • WB: K562 and VCAM1 transfected HEK-293T whole cell lysate. IHC: Human breast cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab227386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/20000. Predicted molecular weight: 81 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
  • Tissue specificity

    Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
  • Sequence similarities

    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
  • Domain

    Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
  • Post-translational
    modifications

    Sialoglycoprotein.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD106 antibody
    • CD106 Antigen antibody
    • INCAM 100 antibody
    • INCAM-100 antibody
    • L1CAM antibody
    • MGC99561 antibody
    • V-CAM 1 antibody
    • Vascular Cell Adhesion Molecule 1 antibody
    • Vascular cell adhesion protein 1 antibody
    • VCAM 1 antibody
    • VCAM-1 antibody
    • VCAM1 antibody
    • VCAM1_HUMAN antibody
    see all

Images

  • Paraffin-embedded human breast cancer tissue stained for VCAM1 using ab227386 at 1/250 dilution in immunohistochemical analysis.

  • Anti-VCAM1 antibody - N-terminal (ab227386) at 1/5000 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 81 kDa

  • All lanes : Anti-VCAM1 antibody - N-terminal (ab227386) at 1/20000 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : VCAM1 transfected HEK-293T whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 81 kDa

References

ab227386 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227386.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up