Application
Western blot
Sample
Caenorhabditis elegans Tissue lysate - whole (whole worm lysate)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
20 µg
Specification
whole worm lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST
Dilution
1/3000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Donkey anti Rabbit IRDye 800CW
Host species: Donkey
Clonality: Monoclonal
Conjugation: IRDye® 800CW
Host species: Donkey
Clonality: Monoclonal
Conjugation: IRDye® 800CW
Detection
Detection method
Fluorescent
Negative control
VCP RNAi treated worm lysate
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 95 kDa Non-specific: 80, 130 kDa
Positive control
Wild type worm lysate
Additional data
Additional Notes
1. wild type
2. VCP (cdc48.1, cdc48.2 double) RNAi treated
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Nan Xin
Verified customer
Submitted May 08 2019