Recombinant Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10852(B)] to VDAC1/Porin + VDAC2 - Mitochondrial Loading Control
- Suitable for: WB, IHC-P, ICC/IF, IHC-Fr
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control -
Description
Rabbit monoclonal [EPR10852(B)] to VDAC1/Porin + VDAC2 - Mitochondrial Loading Control -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2, Jurkat, HEK-293, HAP1 and HeLa cell lysates; Mouse and rat kidney lysate; Rat cerebellum whole tissue lysate IHC-P: Human liver, heart, kidney, ovarian carcinoma, thyroid gland carcinoma, skeletal muscle and cervical carcinoma tissues; Rat kidney tissue; Mouse cardiac muscle tissue; ICC/IF: HeLa and Jurkat cells; IHC-Fr: Mouse cardiac and skeletal muscle tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10852(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154856 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (4) |
1/1000 - 1/10000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/1000.
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IHC-Fr |
1/50.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) |
Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/1000. |
IHC-Fr
1/50. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) |
Target
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Cellular localization
VDAC1/Porin: Mitochondrion outer membrane. Cell membrane. VDAC2: Mitochondrion outer membrane. -
Database links
- Entrez Gene: 7416 Human
- Entrez Gene: 7417 Human
- Entrez Gene: 22333 Mouse
- Entrez Gene: 22334 Mouse
- Entrez Gene: 83529 Rat
- Entrez Gene: 83531 Rat
- Omim: 193245 Human
- Omim: 604492 Human
see all
Images
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Lanes 1-3 : Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856) at 1/1000 dilution
Lanes 4-5 : Anti-GST antibody [EPR4236] (ab111947) at 1/1000 dilution
Lane 6 : Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) at 1/1000 dilution
Lanes 1 & 4 : N-GST tagged full length recombinant human VDAC1 protein 10ng
Lanes 2 & 5 : N-GST tagged full length recombinant human VDAC2 protein 10ng
Lanes 3 & 6 : C-His tagged full length Recombinant Human VDAC3 protein 10ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 55, 33 kDa why is the actual band size different from the predicted?
Exposure time: 40 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
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Immunohistochemistry (Frozen sections) analysis of mouse skeletal muscle tissue sections labeling VDAC1 / Porin with Purified ab154856 at 1/50 (0.7 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.
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Immunohistochemical staining of paraffin embedded human cervical carcinoma with purified ab154856 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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All lanes : Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856) at 1/10000 dilution (purified)
Lane 1 : HepG2 cell lysate
Lane 2 : HEK293 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
ab154856 staining VDAC1 / Porin showing cytoplasmic staining in HeLa cells (Human cervix adenocarcinoma epithelial cells) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol, Samples were incubated with primary antibody (1/1000) for 1 hour at 21°C. ab150077, an Alexa Fluor® 488-conjugated Goat anti-Rabbit IgG (1:1000) was used as the secondary antibody. DAPI (1/200) was used as a counter stain.
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All lanes : Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856) at 1/10000 dilution (purified)
Lane 1 : mouse kidney lysate
Lane 2 : rat kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
Immunohistochemistry (Frozen sections) analysis of mouse cardiac muscle tissue sections labeling VDAC1 / Porin with Purified ab154856 at 1/50 (0.7 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.
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All lanes : Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856) at 1/1000 dilution (unpurified)
Lane 1 : HepG2 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 31 kDaSecondary antibody - anti-rabbit HRP (ab6721)
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ab154856 staining VDAC1 / Porin showing cytoplasmic staining in Jurkat cells (Human T cell leukemia T lymphocyte) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol, Samples were incubated with primary antibody (1/1000) for 1 hour at 21°C. ab150077, an Alexa Fluor® 488-conjugated Goat anti-Rabbit IgG (1:1000) was used as the secondary antibody. DAPI (1/200) was used as a counter stain.
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Immunohistochemical staining of paraffin embedded rat kidney with purified ab154856 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Immunohistochemical staining of paraffin embedded mouse cardiac muscle with purified ab154856 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856) at 1/2000 dilution (purified) + Jurkat cell lysate at 20 µg
Secondary
HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856) at 1/5000 dilution (unpurified) + Rat cerebellum whole tissue lysate at 30 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97069) (undiluted)
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 2 seconds
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Immunohistochemical analysis of paraffin-embedded human liver tissue labeling VDAC1 with unpurified ab154856 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human heart tissue labeling VDAC1 with unpurified ab154856 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded human normal kidney tissue using unpurified ab154856 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded human ovarian carcinoma tissue using unpurified ab154856 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded human thyroid gland carcinoma tissue using unpurified ab154856 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded human skeletal muscle tissue using unpurified ab154856 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (79)
ab154856 has been referenced in 79 publications.
- Wang J et al. Extensive mitochondrial proteome disturbance occurs during the early stages of acute myocardial ischemia. Exp Ther Med 23:85 (2022). PubMed: 34938367
- Meng F et al. Lysosomal iron recycling in mouse macrophages is dependent upon both LcytB and Steap3 reductases. Blood Adv 6:1692-1707 (2022). PubMed: 34982827
- Lotlikar MS et al. Microfluidic separation of axonal and somal compartments of neural progenitor cells differentiated in a 3D matrix. STAR Protoc 3:101028 (2022). PubMed: 35059649
- Mise S et al. Kastor and Polluks polypeptides encoded by a single gene locus cooperatively regulate VDAC and spermatogenesis. Nat Commun 13:1071 (2022). PubMed: 35228556
- Tayyeb A et al. Calreticulin Shortage Results in Disturbance of Calcium Storage, Mitochondrial Disease, and Kidney Injury. Cells 11:N/A (2022). PubMed: 35456008