Western blot abreview for Anti-VDAC1 / Porin antibody [EPR10852(B)] - Mitochondrial Loading Control

Excellent
Abreviews
Application
Western blot
Sample
Human Tissue lysate - whole (Placenta)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
25 µg
Specification
Placenta
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBST
Dilution
1/1250

Secondary antibody

Dilution
1/20000
Name
Non-Abcam antibody was used: Goat Anti-Rabbit Invitrogen 65-6120
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 31 kDa

Dr. Polina Vishnyakova

Verified customer

Submitted Oct 01 2015

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