Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)
- Datasheet
- References (174)
- Protocols
Overview
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Product nameAnti-VDAC1 / Porin antibody - Mitochondrial Loading Control
See all VDAC1 / Porin primary antibodies -
DescriptionRabbit polyclonal to VDAC1 / Porin - Mitochondrial Loading Control
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Host speciesRabbit
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SpecificityThis antibody detects VDAC1, VDAC2 and VDAC3.
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Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Chicken, Dog, Human, Zebrafish, Chinese hamster
Predicted to work with: Rabbit, Cow, Pig
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Immunogen
Synthetic peptide corresponding to Human VDAC1/ Porin aa 150-250. The immunogen sequence is completely conserved between VDAC1, VDAC2 and VDAC3
(Peptide available asab16131) -
Positive control
- This antibody gave a positive control in the following human whole cell lysates: HeLa A431 Jurkat HEK293 This antibody gave a positive control in HeLa (Human epithelial carcinoma cell line) Nuclear This antibody gave a positive control in the following mouse tissue lysates: Heart Kidney Skeletal Muscle Spinal Cord This antibody gave a positive control in the following rat lysates: PC12 Whole Cell Brain Tissue Lysate - normal tissue Kidney Whole Cell Lysate - normal tissue
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab15895 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa). Abcam recommends using BSA as the blocking agent. |
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| IHC-P | Use at an assay dependent concentration. | |
| ICC/IF | Use a concentration of 5 µg/ml. |
Target
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FunctionForms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
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Tissue specificityHeart, liver and skeletal muscle.
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Sequence similaritiesBelongs to the eukaryotic mitochondrial porin family.
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DomainConsists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity.
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Cellular localizationMitochondrion outer membrane. Cell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 416320 Chicken
- Entrez Gene: 282119 Cow
- Entrez Gene: 7416 Human
- Entrez Gene: 22333 Mouse
- Entrez Gene: 397010 Pig
- Entrez Gene: 83529 Rat
- Entrez Gene: 334582 Zebrafish
- Omim: 604492 Human
see all -
Alternative names
- hVDAC1 antibody
- mVDAC5 antibody
- OMP2 antibody
see all
Images
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Western blot - Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)All lanes : Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895) at 1 µg/ml
Lane 1 : Hela Nuclear lysate
Lane 2 : Hela cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : HEK293 cell lysate
Lane 6 : Hela Nuclear lysate with Human VDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lane 7 : Hela cell lysate with Human VDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lane 8 : A431 cell lysate with Human VDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lane 9 : Jurkat cell lysate with Human VDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lane 10 : HEK293 cell lysate with Human VDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 31 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)Image courtesy of Human Protein AtlasParaffin embedded sections of human kidney were incubated with ab15895 (1/600 dilution) at room temperature for 30 mins. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab15895 was also tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further images can be found at www.proteinatlas.org
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Immunocytochemistry/ Immunofluorescence - Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)ab15895 staining VDAC1/Porin HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab15895 at 5ugml then detected with an Alexa Fluor® 488 goat anti-rabbit secondary antibody (ab150081) at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue), and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)Image courtesy of Carl Hobbs by Abreviewab15895 staining VDAC1/Porin in Human Testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/50 in TBS/BSA/azide buffer) for 16 hours at 21°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.
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Western blot - Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)All lanes : Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895) at 1 µg/ml
Lane 1 : Heart (Mouse) Tissue Lysate
Lane 2 : Kidney (Mouse) Tissue Lysate
Lane 3 :Mouse skeletal muscle tissue lysate - total protein (ab29711)
Lane 4 : Spinal Cord (Mouse) Tissue Lysate
Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 6 : Brain (Rat) Tissue Lysate - normal tissue
Lane 7 : Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 31 kDa -
Western blot - Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)This image is courtesy of an anonymous Abreviewab15895 staining mouse heart tissue section by IHC-P. Sections were PFA fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking in 10% serum for 10 minutes at 25°C. The primary antibody was diluted 1/600 and incubated with the sample for 1 hour at 25°C. A biotinylated goat anti-rabbit antibody diluted 1/400 was used as the secondary.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)Image courtesy of Dr Markus Kipp by Abreview.ab15895 staining VDAC1 / Porin in human cerebellum tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed in paraformaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then incubated with ab15895 at a 1/1000 dilution for 12 hours at 4°C. The secondary used was an undiluted HRP conjugated anti-rabbit polyclonal. Note: The big cells are purkinje cells of the cerebellum, (no nuclear staining). -
Western blot - Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)Western blot image using the Optiblot Reducing Electrophoresis Kit - 10 x 10 cm (4-20%) (ab119220) with the
Prism Ultra Protein Ladder (ab116028) 5µl used. We recommend using our ECL substrate kit (ab65623) .
20ug of Lysate per lane and detection using ab15895 diluted to 1ug/ml.
Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: A431 cell lysate
Lane 4: HEK293 cell lysate
Lane 5: HepG2 cell lysate.
Protocols
References
This product has been referenced in:
- Yeo D et al. Intensified mitophagy in skeletal muscle with aging is downregulated by PGC-1alpha overexpression in vivo. Free Radic Biol Med 130:361-368 (2019). Read more (PubMed: 30395971) »
- McWilliams TG et al. Phosphorylation of Parkin at serine 65 is essential for its activation in vivo. Open Biol 8:N/A (2018). Read more (PubMed: 30404819) »
