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    vdac1-porin-antibody-mitochondrial-loading-control-ab15895.pdf

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Tags & Cell Markers Subcellular Markers Organelles Mitochondria
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Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)

  • Datasheet
  • SDS
Reviews (34)Q&A (3)References (231)

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Western blot - Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)
  • Immunocytochemistry/ Immunofluorescence - Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)
  • Western blot - Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)
  • Western blot - Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)
  • Western blot - Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)

Key features and details

  • Rabbit polyclonal to VDAC1 / Porin - Mitochondrial Loading Control
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Chicken, Dog, Human, Chinese hamster
  • Isotype: IgG

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Overview

  • Product name

    Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control
    See all VDAC1 / Porin primary antibodies
  • Description

    Rabbit polyclonal to VDAC1 / Porin - Mitochondrial Loading Control
  • Host species

    Rabbit
  • Specificity

    This antibody detects VDAC1, VDAC2 and VDAC3.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Mouse
    Chicken
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human VDAC1/ Porin aa 150-250. The immunogen sequence is completely conserved between VDAC1, VDAC2 and VDAC3
    (Peptide available as ab16131)

  • Positive control

    • This antibody gave a positive control in the following human whole cell lysates: HeLa A431 Jurkat HEK293 This antibody gave a positive control in HeLa (Human epithelial carcinoma cell line) Nuclear This antibody gave a positive control in the following mouse tissue lysates: Heart Kidney Skeletal Muscle Spinal Cord This antibody gave a positive control in the following rat lysates: PC12 Whole Cell Brain Tissue Lysate - normal tissue Kidney Whole Cell Lysate - normal tissue

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Isotype/Loading Controls
    • Loading Controls
    • VDAC1 - Mitochondrial Preps
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab15895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Mouse
Chicken
Human
All applications
Rabbit
Cow
Pig
Zebrafish
Application Abreviews Notes
WB (19)
Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).

Abcam recommends using BSA as the blocking agent.

ICC/IF (1)
Use a concentration of 5 µg/ml.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).

Abcam recommends using BSA as the blocking agent.

ICC/IF
Use a concentration of 5 µg/ml.

Target

  • Function

    Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
  • Tissue specificity

    Heart, liver and skeletal muscle.
  • Sequence similarities

    Belongs to the eukaryotic mitochondrial porin family.
  • Domain

    Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity.
  • Cellular localization

    Mitochondrion outer membrane. Cell membrane.
  • Target information above from: UniProt accession P21796 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 416320 Chicken
    • Entrez Gene: 282119 Cow
    • Entrez Gene: 7416 Human
    • Entrez Gene: 22333 Mouse
    • Entrez Gene: 397010 Pig
    • Entrez Gene: 83529 Rat
    • Entrez Gene: 334582 Zebrafish
    • Omim: 604492 Human
    • SwissProt: P45879 Cow
    • SwissProt: P21796 Human
    • SwissProt: Q60932 Mouse
    • SwissProt: Q9MZ16 Pig
    • SwissProt: Q9TT15 Rabbit
    • SwissProt: Q9Z2L0 Rat
    • SwissProt: Q6NWC1 Zebrafish
    • Unigene: 519320 Human
    • Unigene: 709372 Human
    • Unigene: 3555 Mouse
    • Unigene: 470023 Mouse
    • Unigene: 54594 Rat
    see all
  • Alternative names

    • hVDAC1 antibody
    • mVDAC5 antibody
    • OMP2 antibody
    • Outer mitochondrial membrane protein porin 1 antibody
    • Plasmalemmal porin antibody
    • Porin 31HL antibody
    • Porin 31HM antibody
    • Porin antibody
    • VDAC 1 antibody
    • VDAC 5 antibody
    • VDAC antibody
    • VDAC-1 antibody
    • Vdac1 antibody
    • VDAC1_HUMAN antibody
    • Voltage Dependent Anion Channel 1 antibody
    • Voltage dependent anion selective channel protein 1 antibody
    • Voltage-dependent anion-selective channel protein 1 antibody
    see all

Images

  • Western blot - Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)
    Western blot - Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)
    All lanes : Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895) at 1 µg/ml

    Lane 1 : Hela Nuclear lysate
    Lane 2 : Hela cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : Jurkat cell lysate
    Lane 5 : HEK293 cell lysate
    Lane 6 : Hela Nuclear lysate with Human VDAC1 / Porin peptide (ab16131) at 1 µg/ml
    Lane 7 : Hela cell lysate with Human VDAC1 / Porin peptide (ab16131) at 1 µg/ml
    Lane 8 : A431 cell lysate with Human VDAC1 / Porin peptide (ab16131) at 1 µg/ml
    Lane 9 : Jurkat cell lysate with Human VDAC1 / Porin peptide (ab16131) at 1 µg/ml
    Lane 10 : HEK293 cell lysate with Human VDAC1 / Porin peptide (ab16131) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 31 kDa
    Observed band size: 31 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)
    Immunocytochemistry/ Immunofluorescence - Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)

    ab15895 stained in HeLa cells. Cells were fixed with 100% methanol (5 min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab15895 at 5µg/ml and ab7291 (Mouse monoclonal to alpha Tubulin - Loading Control) used at a 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed, (pseudo-colored green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) preadsorbed, (colored red), both used at a 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.

  • Western blot - Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)
    Western blot - Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)
    All lanes : Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895) at 1 µg/ml

    Lane 1 : Heart (Mouse) Tissue Lysate
    Lane 2 : Kidney (Mouse) Tissue Lysate
    Lane 3 : Mouse skeletal muscle tissue lysate - total protein (ab29711)
    Lane 4 : Spinal Cord (Mouse) Tissue Lysate
    Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 6 : Brain (Rat) Tissue Lysate - normal tissue
    Lane 7 : Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 31 kDa
    Observed band size: 31 kDa

  • Western blot - Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)
    Western blot - Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)
    All lanes : Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895) at 1 µg/ml

    Lane 1 : Chicken liver cell lysate
    Lane 2 : CHO K1 cell lysate
    Lane 3 : MDCK cell lysate
    Lane 4 : Chicken liver cell lysate with Human VDAC1 / Porin peptide (ab16131) at 1 µg/ml
    Lane 5 : CHO K1 cell lysate with Human VDAC1 / Porin peptide (ab16131) at 1 µg/ml
    Lane 6 : MDCK cell lysate with Human VDAC1 / Porin peptide (ab16131) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Alexa fluor goat polyclonal anti-Rabbit IgG at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 31 kDa

  • Western blot - Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)
    Western blot - Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)

    Western blot image using the Optiblot Reducing Electrophoresis Kit - 10 x 10 cm (4-20%) (ab119220) with the
    Prism Ultra Protein Ladder (ab116028) 5µl used. We recommend using our ECL substrate kit (ab65623) .
    20ug of Lysate per lane and detection using ab15895 diluted to 1ug/ml.
    Lane 1: HeLa cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: A431 cell lysate
    Lane 4: HEK293 cell lysate
    Lane 5: HepG2 cell lysate.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (231)

    Publishing research using ab15895? Please let us know so that we can cite the reference in this datasheet.

    ab15895 has been referenced in 231 publications.

    • Wang J  et al. Fundc1-dependent mitophagy is obligatory to ischemic preconditioning-conferred renoprotection in ischemic AKI via suppression of Drp1-mediated mitochondrial fission. Redox Biol 30:101415 (2020). PubMed: 31901590
    • Tian F  et al. miR-210 in Exosomes Derived from Macrophages under High Glucose Promotes Mouse Diabetic Obesity Pathogenesis by Suppressing NDUFA4 Expression. J Diabetes Res 2020:6894684 (2020). PubMed: 32258168
    • Fabbri L  et al. Identification of a new aggressive axis driven by ciliogenesis and absence of VDAC1-?C in clear cell Renal Cell Carcinoma patients. Theranostics 10:2696-2713 (2020). PubMed: 32194829
    • Matsubara H  et al. The human Bcl-2 family member Bcl-rambo and voltage-dependent anion channels manifest a genetic interaction in Drosophila and cooperatively promote the activation of effector caspases in human cultured cells. Exp Cell Res 381:223-234 (2019). PubMed: 31102594
    • Gonzalez-Armenta JL  et al. Skeletal Muscle Mitochondrial Respiration Is Elevated in Female Cynomolgus Macaques Fed a Western Compared with a Mediterranean Diet. J Nutr 149:1493-1502 (2019). PubMed: 31112997
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-10 of 34 Abreviews

    Western blot abreview for Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (kidney)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Loading amount
    40 µg
    Specification
    kidney
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 02 2021

    Immunohistochemistry (Frozen sections) abreview for Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (kidney cortex)
    Permeabilization
    No
    Specification
    kidney cortex
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 02 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (1,(C) VDAC1 and (B) overlaid image of kidney tubul)
    Permeabilization
    Yes - 0.02% Triton X
    Specification
    1,(C) VDAC1 and (B) overlaid image of kidney tubul
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 27 2021

    Western blot abreview for Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Dopaminergic Neurons)
    Gel Running Conditions
    Reduced Denaturing (NuPage 4-12% MES buffer)
    Loading amount
    30 µg
    Specification
    Dopaminergic Neurons
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 22 2020

    Western blot abreview for Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (colon cancer, kidney)
    Gel Running Conditions
    Reduced Denaturing (4-12% bis-tris gel)
    Loading amount
    30 µg
    Specification
    colon cancer, kidney
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 09 2020

    Western blot abreview for Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Breast cancer cell line)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    25 µg
    Specification
    Breast cancer cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 15 2020

    Western blot abreview for Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Chinese hamster Cell lysate - whole cell (CHO cells (Chinese hamster ovary))
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    25 µg
    Specification
    CHO cells (Chinese hamster ovary)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Herr Dr. Vladimir Milenkovic

    Verified customer

    Submitted Oct 28 2019

    Western blot abreview for Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Huh7, hepatocyte-derived carcinoma cell line)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    25 µg
    Specification
    Huh7, hepatocyte-derived carcinoma cell line
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Herr Dr. Vladimir Milenkovic

    Verified customer

    Submitted Oct 28 2019

    Flow Cytometry abreview for Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (fibroblasts)
    Permeabilization
    Yes - saponin
    Gating Strategy
    single cells
    Specification
    fibroblasts
    Preparation
    Cell harvesting/tissue preparation method: trypsin
    Sample buffer: FACS buffer
    Fixation
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 27 2019

    Western blot abreview for Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - other (Fractional from primary endothelial cell)
    Gel Running Conditions
    Reduced Denaturing (10% SDS gel)
    Loading amount
    1 µg
    Specification
    Fractional from primary endothelial cell
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Dr. PeiYi Su

    Verified customer

    Submitted Apr 09 2018

    1-10 of 34 Abreviews

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