Overview

  • Product name

  • Description

    Rabbit polyclonal to VDAC1/Porin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human VDAC1/Porin (N terminal).
    Database link: P21796

  • Positive control

    • WB: HeLa whole cell lysate. ICC/IF: RKO cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab223570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).

Target

  • Function

    Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
  • Tissue specificity

    Heart, liver and skeletal muscle.
  • Sequence similarities

    Belongs to the eukaryotic mitochondrial porin family.
  • Domain

    Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity.
  • Cellular localization

    Mitochondrion outer membrane. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • N2441 antibody
    • OMP2 antibody
    • POR1 antibody
    • hVDAC1 antibody
    • MGC111064 antibody
    • Mitochondrial Porin antibody
    • Outer mitochondrial membrane protein porin 1 antibody
    • Plasmalemmal porin antibody
    • Porin 31HL antibody
    • Porin 31HM antibody
    • VDAC antibody
    • VDAC-1 antibody
    • Vdac1 antibody
    • VDAC1_HUMAN antibody
    • Voltage dependent anion channel 1 antibody
    • Voltage dependent anion selective channel protein 1 antibody
    • Voltage-dependent anion-selective channel protein 1 antibody
    • YNL055C antibody
    • YNL2441C antibody
    see all

Images

  • Anti-VDAC1/Porin antibody (ab223570) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Secondary
    Goat Anti-Rabbit IgG HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 31 kDa
    Observed band size: 31 kDa


    Exposure time: 6 minutes


    Blocking buffer: 5% skim milk in TBST.

    Primary antibody was incubated for 2 hours, the secondary for 1 hour at RT.

  • 4% formaldehyde-fixed RKO cells stained for VDAC1/Porin (green) using ab223570 at 1/100 dilution in ICC/IF. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1/100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 minutes RT, 5 minutes RT.

    (A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223570 (D) Merge.

     

References

ab223570 has not yet been referenced specifically in any publications.

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