Key features and details
- Rabbit polyclonal to VDAC1/Porin
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-VDAC1/Porin antibody
See all VDAC1/Porin primary antibodies
DescriptionRabbit polyclonal to VDAC1/Porin
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human VDAC1/Porin (N terminal).
Database link: P21796
- WB: HeLa whole cell lysate. ICC/IF: RKO cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab223570 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).|
FunctionForms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
Tissue specificityHeart, liver and skeletal muscle.
Sequence similaritiesBelongs to the eukaryotic mitochondrial porin family.
DomainConsists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity.
Cellular localizationMitochondrion outer membrane. Cell membrane.
- Information by UniProt
- N2441 antibody
- OMP2 antibody
- POR1 antibody
Anti-VDAC1/Porin antibody (ab223570) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Goat Anti-Rabbit IgG HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 6 minutes
Blocking buffer: 5% skim milk in TBST.
Primary antibody was incubated for 2 hours, the secondary for 1 hour at RT.
4% formaldehyde-fixed RKO cells stained for VDAC1/Porin (green) using ab223570 at 1/100 dilution in ICC/IF. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1/100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 minutes RT, 5 minutes RT.
(A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223570 (D) Merge.
ab223570 has not yet been referenced specifically in any publications.