Overview

  • Product name

  • Description

    Goat polyclonal to VDAC1/Porin
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • WB: Recombinant human VDAC1 protein; Mouse heart lysate; HeLa cell lysate; Rat brain lysate. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab235143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 31 kDa.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
  • Tissue specificity

    Heart, liver and skeletal muscle.
  • Sequence similarities

    Belongs to the eukaryotic mitochondrial porin family.
  • Domain

    Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity.
  • Cellular localization

    Mitochondrion outer membrane. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • N2441 antibody
    • OMP2 antibody
    • POR1 antibody
    • hVDAC1 antibody
    • MGC111064 antibody
    • Mitochondrial Porin antibody
    • Outer mitochondrial membrane protein porin 1 antibody
    • Plasmalemmal porin antibody
    • Porin 31HL antibody
    • Porin 31HM antibody
    • VDAC antibody
    • VDAC-1 antibody
    • Vdac1 antibody
    • VDAC1_HUMAN antibody
    • Voltage dependent anion channel 1 antibody
    • Voltage dependent anion selective channel protein 1 antibody
    • Voltage-dependent anion-selective channel protein 1 antibody
    • YNL055C antibody
    • YNL2441C antibody
    see all

Images

  • Immunofluorescent analysis of 100% methanol fixed, 0.3% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling VDAC1/Porin with ab235143 at 5 µg/ml (overnight at 4°C), followed by an anti-Goat IgG DyLight™488 conjugated preadsorbed secondary antibody at 15 µg/ml (1 hour at RT) (green).
    The nuclear counterstain is DAPI (blue).

    Panel A: DAPI.
    Panel B: ab235143 + secondary antibody.
    Panel C: Merge of panel A and B.
    Panel D: Secondary antibody only.

  • All lanes : Anti-VDAC1/Porin antibody (ab235143) at 1/1000 dilution

    Lane 1 : Recombinant VDAC1 protein
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 3 : Rat brain lysate
    Lane 4 : Mouse heart lysate

    Secondary
    All lanes : F(ab)'2 Donkey anti-Goat IgG (H&L)-HRP at 1/70000 dilution

    Predicted band size: 31 kDa


    Exposure time: 60 seconds


    Dilution buffer: SDS.

  • All lanes : Anti-VDAC1/Porin antibody (ab235143) at 1/1000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Mouse heart lysate

    Secondary
    All lanes : F(ab)'2 Donkey anti-Goat IgG (H&L) - HRP at 1/70000 dilution

    Predicted band size: 31 kDa


    Exposure time: 60 seconds


    Blocking/Dilution buffer: Casein.

References

ab235143 has not yet been referenced specifically in any publications.

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