Key features and details
- Rabbit polyclonal to VDAC1/Porin
- Suitable for: IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-VDAC1/Porin antibody
See all VDAC1/Porin primary antibodies
DescriptionRabbit polyclonal to VDAC1/Porin
SpecificityCross reactivity will occur with all forms of VDACs including VDAC1, VDAC2 (4 isoforms) and VDAC3(2 isoforms). This broad reactivity makes ab34726 useful as a loading control (mitochondrial).
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Rabbit, Cow, Pig, Zebrafish
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was affinity purified from monospecific antiserum.
Our Abpromise guarantee covers the use of ab34726 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionForms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
Tissue specificityHeart, liver and skeletal muscle.
Sequence similaritiesBelongs to the eukaryotic mitochondrial porin family.
DomainConsists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity.
Cellular localizationMitochondrion outer membrane. Cell membrane.
- Information by UniProt
- N2441 antibody
- OMP2 antibody
- POR1 antibody
IHC image of ab34726 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab34726, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab34726 has been referenced in 34 publications.
- Zhang F et al. P53 and Parkin co-regulate mitophagy in bone marrow mesenchymal stem cells to promote the repair of early steroid-induced osteonecrosis of the femoral head. Cell Death Dis 11:42 (2020). PubMed: 31959744
- León-Aparicio D et al. Novel Potassium Channels in Kidney Mitochondria: The Hyperpolarization-Activated and Cyclic Nucleotide-Gated HCN Channels. Int J Mol Sci 20:N/A (2019). PubMed: 31601020
- Hu D et al. Alpha-synuclein suppresses mitochondrial protease ClpP to trigger mitochondrial oxidative damage and neurotoxicity. Acta Neuropathol 137:939-960 (2019). PubMed: 30877431
- Li XX et al. Nuclear Receptor Nur77 Facilitates Melanoma Cell Survival under Metabolic Stress by Protecting Fatty Acid Oxidation. Mol Cell 69:480-492.e7 (2018). PubMed: 29395065
- Chun SK et al. Loss of sirtuin 1 and mitofusin 2 contributes to enhanced ischemia/reperfusion injury in aged livers. Aging Cell N/A:e12761 (2018). PubMed: 29774638