Overview

  • Product name

  • Description

    Rabbit polyclonal to VDAC1/Porin
  • Host species

    Rabbit
  • Specificity

    Cross reactivity will occur with all forms of VDACs including VDAC1, VDAC2 (4 isoforms) and VDAC3(2 isoforms). This broad reactivity makes ab34726 useful as a loading control (mitochondrial).
  • Tested applications

    Suitable for: ELISA, WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Rabbit, Cow, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human VDAC1/Porin aa 185-197.
    Sequence:

    NDGTEFGGSIYQK

  • Positive control

    • WB: rat heart whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab34726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000 - 1/50000.
WB 1/500 - 1/3000. Detects a band of approximately 32 kDa (predicted molecular weight: 31 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100.

Fixation with PFA requires a denaturing step, see Abreview by M.Schrader.

Target

  • Function

    Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
  • Tissue specificity

    Heart, liver and skeletal muscle.
  • Sequence similarities

    Belongs to the eukaryotic mitochondrial porin family.
  • Domain

    Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity.
  • Cellular localization

    Mitochondrion outer membrane. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • N2441 antibody
    • OMP2 antibody
    • POR1 antibody
    • hVDAC1 antibody
    • MGC111064 antibody
    • Mitochondrial Porin antibody
    • Outer mitochondrial membrane protein porin 1 antibody
    • Plasmalemmal porin antibody
    • Porin 31HL antibody
    • Porin 31HM antibody
    • VDAC antibody
    • VDAC-1 antibody
    • Vdac1 antibody
    • VDAC1_HUMAN antibody
    • Voltage dependent anion channel 1 antibody
    • Voltage dependent anion selective channel protein 1 antibody
    • Voltage-dependent anion-selective channel protein 1 antibody
    • YNL055C antibody
    • YNL2441C antibody
    see all

Images

  • IHC image of ab34726 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab34726, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab34726 at a 1/100 dilution staining VDAC1/Porin in COS7 cells by Immunocytochemistry/ Immunofluorescence.The antibody does not work with a standard fixation for ICC/IF as it does not recognize the non-denatured protein. However, applying a fixation for hidden antigens (modified from Peränen J, Rikkonen M, Kääriäinen L. A method for exposing hidden antigenic sites in paraformaldehyde-fixed cultured cells, applied to initially unreactive antibodies. J Histochem Cytochem. 1993 Mar41(3):447-54) results in very good recognition of VDAC/porin. Denaturing is achieved by Guanidine hydrochloride in 50 mM Tris-HCL pH 7.5 after fixation (3% pFA, 0.05% GA) and permeabilization (0.2% TX-100). The secondary used was a TRITC conjugated donkey anti-rabbit used at a 1/100 dilution.

References

This product has been referenced in:

  • Chun SK  et al. Loss of sirtuin 1 and mitofusin 2 contributes to enhanced ischemia/reperfusion injury in aged livers. Aging Cell N/A:e12761 (2018). Read more (PubMed: 29774638) »
  • Chen Z  et al. Hyperoside alleviates adriamycin-induced podocyte injury via inhibiting mitochondrial fission. Oncotarget 8:88792-88803 (2017). Read more (PubMed: 29179476) »
See all 28 Publications for this product

Customer reviews and Q&As

11-13 of 13 Abreviews or Q&A

Application
Immunocytochemistry
Sample
African Green Monkey Cultured Cells (COS-7, kidney epithelium)
Specification
COS-7, kidney epithelium
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton-X-100
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 20°C

Dr. Michael Schrader

Verified customer

Submitted Feb 18 2010

Application
Western blot
Sample
Rat Cell lysate - whole cell (AR42J cells, pancreatic acinar cells, rat)
Loading amount
20 µg
Specification
AR42J cells, pancreatic acinar cells, rat
Gel Running Conditions
Reduced Denaturing (12.5%, stacking gel: 5%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Dr. Michael Schrader

Verified customer

Submitted Aug 24 2009

Application
Immunocytochemistry/ Immunofluorescence
Sample
African Green Monkey Cell (COS-7, kidney epithelium)
Specification
COS-7, kidney epithelium
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton-X-100
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 20°C

Dr. Michael Schrader

Verified customer

Submitted Jul 13 2009

11-13 of 13 Abreviews or Q&A

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