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What percentage of Guanidine HCl should be used to prepare fixed cells for ICC and how long should they be treated for?
Asked on Oct 13 2010
Thank you for your inquiry. Cells should be permeabilized with Triton X-100 and then incubated in 6 N Guanidine HCl in 50mM Tris-HCl, pH 7.5 for 10 minutes at room temperature. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Oct 13 2010