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We have used the ab34726 VDAC antibody for Western blotting on rat heart
mitochondria in our lab for several years and have always found it to be a
very reliable antibody until we purchased a new batch on 14 August 2012
(Purchase Order FMVS1176576), which despite several attempts on several
preparations of mitochondria and using the antibody at a dilution as low as
1/500 (where previously it has worked fine at 1/3000) have failed to get
any decent bands around the 32kDa area (or at any other molecular weight!).
The antibody we were sent is: LOT GR95237-1. Have you had any other
customers reporting problems with this? Can you please send us a
replacement antibody of a different lot number as we know this antibody
usually works well and can only assume there is something not quite right
with the batch we were sent?
Thank you for your help,
Asked on Sep 11 2012
Thank you for contacting us.
Although other lots of this antibody have worked well under these former conditions, all individual antibodies will require individual optimization.
According to the datasheet, 1/500 is within the recommended dilution range. It may be that this lot has a lower affinity asthe previous ones and therefore need a higher concentration. As the antibody is workingwithin the givenparameters,I am afraid I cannot send out a replacement
I hope this information is still helpful to you. Thank you for your understanding and cooperation.
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Answered on Sep 12 2012