own sample and simultaneous detection of Vimentin in other colour channel
I tested the VDAC AB with two different lysate loading amounts: 20 ug in lanes 1, 3, & 5 and 30 ug lysate in lanes 2, 4 & 6. The AB was tested at three different concentrations and in two different blocking buffers. In my setting it worked best in milk powder at the concentration of1/400. Unfortunately several bands came up and therefore suggesting that multiplexing is very difficult. In summary, it's a solid, average quality antibody doing it's job but not as good as some other abcam antibodies.
Dr. Alexander Henke
Submitted Aug 03 2011
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