Application
Flow Cytometry
Sample
Human Cell (fibroblasts)
Permeabilization
Yes - saponin
Gating Strategy
single cells
Specification
fibroblasts
Preparation
Cell harvesting/tissue preparation method: trypsin
Sample buffer: FACS buffer
Sample buffer: FACS buffer
Fixation
Paraformaldehyde
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution
1/150
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: Jackson goat andt rb affinity purified
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Additional data
Additional Notes
had been successful with previous lots (different cell type, human ips-cardiomyocytes), this lot was not successful for flow
Abcam response
Abcam has not tested ab15895 in Flow Cytometry and thus can't say how it should have performed. This individual received a credit for testing this antibody in Flow Cytometry as part of our AbTrial program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
Submitted Mar 27 2019