Application
Western blot
Sample
Human Cell lysate - whole cell (Dopaminergic Neurons)
Gel Running Conditions
Reduced Denaturing (NuPage 4-12% MES buffer)
Loading amount
30 µg
Specification
Dopaminergic Neurons
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/2000
Secondary antibody
Dilution
1/20000
Name
Non-Abcam antibody was used: IRDye® 800CW Goat anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
LI-COR Odyssey CLx
Exposure
20 second(s)
Bands
Specific: 30 kDa Non-specific: 22 kDa
Additional data
Additional Notes
human Embryonic Stem Cell-derived - 90 days differentiation
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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Submitted Oct 22 2020