Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (1,(C) VDAC1 and (B) overlaid image of kidney tubul)
Permeabilization
Yes - 0.02% Triton X
Specification
1,(C) VDAC1 and (B) overlaid image of kidney tubul
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/250
Secondary antibody
Dilution
1/2500
Name
Non-Abcam antibody was used: Goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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Submitted Jan 27 2021