Key features and details
- Mouse monoclonal [N152B/23] to VDAC1/Porin (Biotin)
- Suitable for: WB, IHC-P, ICC/IF, IHC-Fr
- Reacts with: Rat
- Conjugation: Biotin
- Isotype: IgG2a
Product nameAnti-VDAC1/Porin antibody [N152B/23] (Biotin)
See all VDAC1/Porin primary antibodies
DescriptionMouse monoclonal [N152B/23] to VDAC1/Porin (Biotin)
SpecificityDoes not cross-react with VDAC2 or VDAC3 (based on KO validation results).
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IHC-Frmore details
Species reactivityReacts with: Rat
Predicted to work with: Mouse, Rabbit, Cow, Human, Pig
Recombinant full length protein corresponding to Human VDAC1/Porin aa 1-283.
MAVPPTYADLGKSARDVFTKGYGFGLIKLDLKTKSENGLEFTSSGSANTE TTKVTGSLET KYRWTEYGLTFTEKWNTDNTLGTEITVEDQLARGLKLT FDSSFSPNTGKKNAKIKTGYKR EHINLGCDMDFDIAGPSIRGALVLGY EGWLAGYQMNFETAKSRVTQSNFAVGYKTDEFQL HTNVNDGTEFGGSI YQKVNKKLETAVNLAWTAGNSNTRFGIAAKYQIDPDACFSAKVNNS SL IGLGYTQTLKPGIKLTLSALLDGKNVNAGGHKLGLGLEFQA
Database link: P21796
- Rat brain lysate.
The clone number has been updated from S152B-23 to N152B/23, both clone numbers name the same antibody clone.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab186262 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 31 kDa).|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
FunctionForms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
Tissue specificityHeart, liver and skeletal muscle.
Sequence similaritiesBelongs to the eukaryotic mitochondrial porin family.
DomainConsists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity.
Cellular localizationMitochondrion outer membrane. Cell membrane.
- Information by UniProt
- N2441 antibody
- OMP2 antibody
- POR1 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab186262 has not yet been referenced specifically in any publications.