Anti-VDAC2 antibody (ab118872)
Key features and details
- Goat polyclonal to VDAC2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-VDAC2 antibody
See all VDAC2 primary antibodies -
Description
Goat polyclonal to VDAC2 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog, Pig -
Immunogen
Synthetic peptide within Human VDAC2 aa 250 to the C-terminus (C terminal) (Cysteine residue). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P45880-1 -
Positive control
- Human testis lysate, Human heart tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 98% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab118872 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 32 kDa.
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IHC-P |
Use a concentration of 2.5 - 5 µg/ml.
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Notes |
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WB
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 32 kDa. |
IHC-P
Use a concentration of 2.5 - 5 µg/ml. |
Target
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Function
Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. -
Tissue specificity
Expressed in all tissues examined. -
Sequence similarities
Belongs to the eukaryotic mitochondrial porin family. -
Domain
Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. -
Cellular localization
Mitochondrion outer membrane. - Information by UniProt
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Database links
- Entrez Gene: 282120 Cow
- Entrez Gene: 479255 Dog
- Entrez Gene: 7417 Human
- Entrez Gene: 22334 Mouse
- Entrez Gene: 397659 Pig
- Entrez Gene: 83531 Rat
- Omim: 193245 Human
- SwissProt: P45880 Human
see all -
Alternative names
- B-36 vdac antibody
- FLJ23841 antibody
- hVDAC2 antibody
see all
Images
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Immunohistochemical staining of ab118872 at 5 µg/ml staining VDAC2 in Paraffin-embedded Human heart tissue followed by biotinylated anti-Goat IgG secondary antibody, alkaline phosphatasestreptavidin and chromogen
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Anti-VDAC2 antibody (ab118872) at 0.3 µg/ml + Human testis lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 32 kDa
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab118872 has been referenced in 2 publications.
- Triplett JC et al. Age-related changes in the proteostasis network in the brain of the naked mole-rat: Implications promoting healthy longevity. Biochim Biophys Acta 1852:2213-24 (2015). PubMed: 26248058
- Tanno M et al. Translocation of glycogen synthase kinase-3ß (GSK-3ß), a trigger of permeability transition, is kinase activity-dependent and mediated by interaction with voltage-dependent anion channel 2 (VDAC2). J Biol Chem 289:29285-96 (2014). PubMed: 25187518