Key features and details
- Rabbit polyclonal to VDAC2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-VDAC2 antibody
See all VDAC2 primary antibodies
DescriptionRabbit polyclonal to VDAC2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment, corresponding to a region within amino acids 1-189 of Human VDAC2 (UniProt ID: P45880).
- A549 HeLa cell lysate; HepG2 and HCT116 whole cell lysates
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab126120 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 32 kDa.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with 10mM Citrate buffer (PH6.0) or Tris-EDTA buffer ( PH8.0)).|
FunctionForms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective.
Tissue specificityExpressed in all tissues examined.
Sequence similaritiesBelongs to the eukaryotic mitochondrial porin family.
DomainConsists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands.
Cellular localizationMitochondrion outer membrane.
- Information by UniProt
- B-36 vdac antibody
- FLJ23841 antibody
- hVDAC2 antibody
All lanes : Anti-VDAC2 antibody (ab126120) at 1/1000 dilution
Lane 1 : HepG2 whole cel lysate
Lane 2 : HCT116 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 32 kDa
12% SDS PAGE
ab126120, at 1/250 dilution, staining VDAC2 in paraffin embedded Human colon carcinoma tissue by Immunohistochemistry.
ab126120 has been referenced in 2 publications.
- Aono Y et al. Sulindac sulfone inhibits the mTORC1 pathway in colon cancer cells by directly targeting voltage-dependent anion channel 1 and 2. Biochem Biophys Res Commun 505:1203-1210 (2018). PubMed: 30327144
- Zhou K et al. VDAC2 interacts with PFKP to regulate glucose metabolism and phenotypic reprogramming of glioma stem cells. Cell Death Dis 9:988 (2018). PubMed: 30250190