Overview

  • Product name

    Anti-VDAC2 antibody - C-terminal
    See all VDAC2 primary antibodies
  • Description

    Rabbit polyclonal to VDAC2 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide, corresponding to a region within C terminal amino acids 233-294 of Human VDAC2 (Uniprot ID P45880)

  • Positive control

    • HepG2 and mouse liver whole cell lysate, methanol-fixed HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab155803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 32 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective.
  • Tissue specificity

    Expressed in all tissues examined.
  • Sequence similarities

    Belongs to the eukaryotic mitochondrial porin family.
  • Domain

    Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands.
  • Cellular localization

    Mitochondrion outer membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • B-36 vdac antibody
    • FLJ23841 antibody
    • hVDAC2 antibody
    • OTTHUMP00000019878 antibody
    • OTTHUMP00000019879 antibody
    • OTTHUMP00000019880 antibody
    • Outer mitochondrial membrane protein porin 2 antibody
    • POR antibody
    • Porin-2 antibody
    • VDAC 2 antibody
    • VDAC-2 antibody
    • VDAC2 antibody
    • VDAC2_HUMAN antibody
    • Vdac6 antibody
    • Voltage dependent anion channel 2 antibody
    • Voltage dependent anion selective channel protein 2 antibody
    • Voltage-dependent anion-selective channel protein 2 antibody
    see all

Images

  • Anti-VDAC2 antibody - C-terminal (ab155803) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg

    Predicted band size: 32 kDa



    12% SDS PAGE
  • Anti-VDAC2 antibody - C-terminal (ab155803) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 32 kDa



    12% SDS PAGE
  • Confocal immunofluorescence analysis of methanol-fixed HeLa cells labeling VDAC2 with ab155803 at 1/500 dilution (green). Alpha-tubulin filament labeling is shown in red.

References

ab155803 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Tissue lysate - other (Mitochondria from cortex, human tissue)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris gradient gel. Laemmli buffer with 10% β-Mercaptoethanol. Samples were denatured at 55C for 10 min. Immobilon-FL transfer membranes. Overnight transfer at +4C.)
Loading amount
15 µg
Specification
Mitochondria from cortex, human tissue
Blocking step
SEA Block Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Aug 23 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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