Western blot abreview for Anti-VDAC3 antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Huh7.5 cell lines)
Gel Running Conditions
Non-reduced Denaturing (10%)
Loading amount
20 µg
Specification
Huh7.5 cell lines
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS Tween + 1% milk
Dilution
1/3000

Secondary antibody

Name
Non-Abcam antibody was used: goat anti rabbit HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
45 second(s)
Bands
Specific: 31 kDa

Additional data

Additional Notes
Validation of the antibody for the isoform 3 of VDAC

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Verified customer

Submitted Jul 11 2019

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