Recombinant Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free (ab232515)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18229] to VE Cadherin - BSA and Azide free
- Suitable for: ICC/IF, IP, WB
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free
See all VE Cadherin primary antibodies -
Description
Rabbit monoclonal [EPR18229] to VE Cadherin - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: bEnd.3 cells.
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General notes
ab232515 is the carrier-free version of ab205336.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18229 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232515 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 125, 90 kDa (predicted molecular weight: 88 kDa).
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 125, 90 kDa (predicted molecular weight: 88 kDa). |
Target
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Function
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. -
Tissue specificity
Endothelial tissues and brain. -
Sequence similarities
Contains 5 cadherin domains. -
Post-translational
modificationsPhosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB. -
Cellular localization
Cell junction. Cell membrane. Found at cell-cell boundaries and probably at cell-matrix boundaries. - Information by UniProt
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Database links
- Entrez Gene: 12562 Mouse
- SwissProt: P55284 Mouse
- Unigene: 21767 Mouse
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Alternative names
- 7B 4 antibody
- 7B4 antibody
- 7B4 antigen antibody
see all
Images
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VE Cadherin was immunoprecipitated from 1mg of Mouse lung whole cell lysate with ab205336 at 1/80 dilution.
Western blot was performed from the immunoprecipitate using ab205336 at 1/1000 dilution.
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.
Lane 1: Mouse lung whole cell lysate 10ug (Input).
Lane 2: ab205336 IP in Mouse lung whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205336 in Mouse lung whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
Due to a high degree of glycosylation and phosphorylation, the observed MW is higher than the predicted MW. The 90kDa fragment represents the extracellular domain where the immunogen is located.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205336).
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VE Cadherin was immunoprecipitated from 1mg of bEnd.3 (Mouse brain microvascular endothelial cell line) whole cell lysate with ab205336 at 1/80 dilution.
Western blot was performed from the immunoprecipitate using ab205336 at 1/1000 dilution.
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.
Lane 1: bEnd.3 whole cell lysate 10ug (Input).
Lane 2: ab205336 IP in bEnd.3 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205336 in bEnd.3 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205336).
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized bEnd.3 (Mouse brain microvascular endothelial cell line) cells labeling VE Cadherin with ab205336 at 1/1000 dilution, followed by Goat anti-rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).
Confocal image showing membrane staining on bEnd.3 cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody -Loading control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (AlexaFluor®594 ) preadsorbed (ab150120) at 1/500 dilution (red).
The negative controls are as follows:-
-ve control 1: ab205336 at 1/1000 dilution followed by ab150120 at 1/500 dilution.
-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205336).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (1)
ab232515 has been referenced in 1 publication.
- Ye Y et al. QiShenYiQi Inhibits Tissue Plasminogen Activator-Induced Brain Edema and Hemorrhage after Ischemic Stroke in Mice. Front Pharmacol 12:759027 (2021). PubMed: 35095486