Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)
- Datasheet
- References (71)
- Protocols
Overview
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Product nameAnti-VE Cadherin antibody - Intercellular Junction Marker
See all VE Cadherin primary antibodies -
DescriptionRabbit polyclonal to VE Cadherin - Intercellular Junction Marker
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, WB, IP, In-Cell ELISA, Flow Cytmore details
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Species reactivityReacts with: Mouse, Chicken, Human
Predicted to work with: Cow, Pig
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Immunogen
Synthetic peptide corresponding to Human VE Cadherin aa 750 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab27462) -
Positive control
- WB: HUVEC Cell Lysate. ICC/IF: HUVEC cells. IHC-P: Mouse heart tissue. Flow Cyt: REH B cells. IP: HUVEC whole cell lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
1x PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Immunizing Peptide (Blocking)
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Related Products
Applications
Our Abpromise guarantee covers the use of ab33168 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| ICC/IF | Use a concentration of 1 - 5 µg/ml. Abcam recommends using this product with confluent cells. |
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| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 115 kDa (predicted molecular weight: 87 kDa).Can be blocked with VE Cadherin peptide (ab27462). Abcam recommends using BSA blocking with this product. Milk blocking will give a greatly reduced signal strength in WB. |
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| IP | Use at an assay dependent concentration. | |
| In-Cell ELISA | Use at an assay dependent concentration. PubMed: 22689949 | |
| Flow Cyt | Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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FunctionCadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton.
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Tissue specificityEndothelial tissues and brain.
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Sequence similaritiesContains 5 cadherin domains.
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Post-translational
modificationsPhosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB. -
Cellular localizationCell junction. Cell membrane. Found at cell-cell boundaries and probably at cell-matrix boundaries.
- Information by UniProt
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Database links
- Entrez Gene: 1003 Human
- Entrez Gene: 12562 Mouse
- Omim: 601120 Human
- SwissProt: P33151 Human
- SwissProt: P55284 Mouse
- Unigene: 76206 Human
- Unigene: 21767 Mouse
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Alternative names
- 7B 4 antibody
- 7B4 antibody
- 7B4 antigen antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)ab33168 stained in HUVEC cells. Cells were fixed with 100% methanol (5min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab33168 at 1µg/ml and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150120 (pseudo-colored red) and ab150081 (colored green) used at 1 ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature
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Western blot - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168) at 1 µg/ml + HUVEC Cell Lysate at 20 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 87 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted?
The band we observe at 115 kDa is believed to be the glycosylated form of the protein. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)This image is courtesy of an anonymous Abreviewab33168 (1/50) staining VE Cadherin in paraffin-embbeded mouse heart tissue sections. Tissue underwent fixation in formaldehyde, heat-mediated antigen retrieval in citrate buffer and blocking (5 minutes/peroxidase block and 10 minutes/protein block). For further experimental details please refer to abreview. -
Flow Cytometry - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)This image is courtesy of an anonymous abreview.ab33168 used in Flow cytometry.
Rabbit IgG isotype control (white)
Human REH B cells were fixed in paraformaldehyde and permeabilized using saponin. Primary antibody used undiluted (2µl in 100µl of cells in PBS) and incubated for 15 minutes at 4°C. The secondary antibody used was an undiluted, Alexa Fluor®488 conjugated goat anti-rabbit IgG. -
Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)This image is courtesy of Stephen Yarwood, Inst Mol, Cell and Sys Bio, United KingdomICC/IF image of VE-Cadherin staining on HUVEC cells using ab33168. The cells were incubated with the primary antibody (ab33168) and the secondary was FITC conjugated anti-rabbit used at 1:400. The cells were incubated with only the secondary antibody as a negative control. -
Immunoprecipitation - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)This image is courtesy of an anonymous Abreviewab33168 Immunoprecipitating VE Cadherin in human HUVEC whole cell lysate. 1000000 cells lysate was incubated with primary antibody (1/100 in 0.5% NP40, 150mM NaCl, 50mM Tris) and matrix (Dynabeads) for 2 hours at 4°C. For western blotting a HRP-conjugated mouse anti-VE Cadherin (1/3000) was used to confirm successful immunoprecipation.
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Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)This image is courtesy of Ana Kasirer-Friede, Univ California-San Diego, Dept. Of Medicine, United StatesICC/IF image of VE Cadherin stained HUVEC cells. The cells were incubated with the antibody ab33168 at 1/150 (Green). The cells were also stained with Rhodamine phalloidin (Red).
Protocols
References
This product has been referenced in:
- Soni D et al. Deubiquitinase function of A20 maintains and repairs endothelial barrier after lung vascular injury. Cell Death Discov 4:60 (2018). Read more (PubMed: 29796309) »
- Miscianinov V et al. MicroRNA-148b Targets the TGF-ß Pathway to Regulate Angiogenesis and Endothelial-to-Mesenchymal Transition during Skin Wound Healing. Mol Ther N/A:N/A (2018). WB, ICC/IF . Read more (PubMed: 29843955) »
