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Signal Transduction Cytoskeleton / ECM Cell Adhesion Cadherins
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Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)

  • Datasheet
  • SDS
Reviews (37)Q&A (15)References (155)

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Western blot - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)
  • Western blot - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)
  • Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)
  • Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)
  • Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)
  • Western blot - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)
  • Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)

Key features and details

  • Rabbit polyclonal to VE Cadherin - Intercellular Junction Marker
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-VE Cadherin antibody - Intercellular Junction Marker
    See all VE Cadherin primary antibodies
  • Description

    Rabbit polyclonal to VE Cadherin - Intercellular Junction Marker
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human VE Cadherin aa 750 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab27462)

  • General notes

      

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    1x PBS
    Batches which are <1mg/ml will contain 1% BSA, batches at 1mg/ml will not.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cadherins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cell Adhesion Molecules
    • Vascular
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cell Adhesion Molecules
    • Endothelial
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Cadherins
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Cell adhesion molecules
    • Cardiovascular
    • Vasculature
    • Endothelium
    • Cardiovascular
    • Cardiovascular Markers
    • Cell Markers
    • Endothelial Cells
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Immunizing Peptide (Blocking)

    • VE Cadherin peptide (ab27462)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Mouse VE Cadherin protein (ab208314)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • Anti-CD31 antibody [P2B1] (ab24590)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab33168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
All applications
Mouse
Chicken
Cow
Pig
Application Abreviews Notes
ICC/IF (17)
Use a concentration of 0.1 - 1 µg/ml.

Abcam recommends using this product with confluent cells.

WB (8)
Use a concentration of 1 µg/ml. Detects a band of approximately 115 kDa (predicted molecular weight: 87 kDa).Can be blocked with VE Cadherin peptide (ab27462).

Abcam recommends using BSA blocking with this product.  Milk blocking will give a greatly reduced signal strength in WB.

Notes
ICC/IF
Use a concentration of 0.1 - 1 µg/ml.

Abcam recommends using this product with confluent cells.

WB
Use a concentration of 1 µg/ml. Detects a band of approximately 115 kDa (predicted molecular weight: 87 kDa).Can be blocked with VE Cadherin peptide (ab27462).

Abcam recommends using BSA blocking with this product.  Milk blocking will give a greatly reduced signal strength in WB.

Target

  • Function

    Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton.
  • Tissue specificity

    Endothelial tissues and brain.
  • Sequence similarities

    Contains 5 cadherin domains.
  • Post-translational
    modifications

    Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB.
  • Cellular localization

    Cell junction. Cell membrane. Found at cell-cell boundaries and probably at cell-matrix boundaries.
  • Target information above from: UniProt accession P33151 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1003 Human
    • Entrez Gene: 12562 Mouse
    • Omim: 601120 Human
    • SwissProt: P33151 Human
    • SwissProt: P55284 Mouse
    • Unigene: 76206 Human
    • Unigene: 21767 Mouse
    • Alternative names

      • 7B 4 antibody
      • 7B4 antibody
      • 7B4 antigen antibody
      • CADH5_HUMAN antibody
      • Cadherin 5 antibody
      • Cadherin 5 type 2 antibody
      • Cadherin 5, type 2 (vascular endothelium) antibody
      • Cadherin 5, type 2, VE cadherin (vascular epithelium) antibody
      • cadherin, vascular endothelial antibody
      • cadherin, vascular endothelial, 1 antibody
      • Cadherin-5 antibody
      • Cadherin5 antibody
      • CD 144 antibody
      • CD144 antibody
      • CD144 antigen antibody
      • CDH 5 antibody
      • CDH5 antibody
      • CDH5 protein antibody
      • Endothelial specific cadherin antibody
      • FLJ17376 antibody
      • OTTHUMP00000174777 antibody
      • Vascular endothelial cadherin antibody
      • Vascular epithelium cadherin antibody
      • VE Cad antibody
      • VE-cadherin antibody
      • VEC antibody
      see all

    Images

    • Western blot - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)
      Western blot - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)
      Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168) at 1 µg/ml + HUVEC Cell Lysate at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 87 kDa
      Observed band size: 120 kDa
      why is the actual band size different from the predicted?


      Exposure time: 1 minute


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab33168 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

       

      The band we observe at 115 kDa is believed to be the glycosylated form of the protein.

    • Western blot - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)
      Western blot - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)
      Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168) at 1 µg/ml + HUVEC Cell Lysate at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 87 kDa
      Observed band size: 120 kDa why is the actual band size different from the predicted?
      Additional bands at: 55 kDa (possible non-specific binding)


      Exposure time: 1 minute


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab33168 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

       

      The band we observe at 115 kDa is believed to be the glycosylated form of the protein.

    • Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)
      Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)This image is courtesy of Stephen Yarwood, Inst Mol, Cell and Sys Bio, United Kingdom
      ICC/IF image of VE-Cadherin staining on HUVEC cells using ab33168. The cells were incubated with the primary antibody (ab33168) and the secondary was FITC conjugated anti-rabbit used at 1:400. The cells were incubated with only the secondary antibody as a negative control.
    • Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)
      Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)

      ab33168 stained in HUVEC cells. Cells were fixed with 100% methanol (5min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab33168 at 1µg/ml and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150120 (pseudo-colored red) and ab150081 (colored green) used at 1 ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature

      This image was generated using confluent cells.

    • Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)
      Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)This image is courtesy of Ana Kasirer-Friede, Univ California-San Diego, Dept. Of Medicine, United States

      ICC/IF image of VE Cadherin stained HUVEC cells. The cells were incubated with the antibody ab33168 at 1/150 (Green). The cells were also stained with Rhodamine phalloidin (Red).

    • Western blot - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)
      Western blot - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)
      Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168) at 1 µg/ml + HUVEC Cell Lysate at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 87 kDa
      Observed band size: 115,117 kDa why is the actual band size different from the predicted?
      Additional bands at: 45 kDa (possible non-specific binding)


      Exposure time: 1 minute


      The observed band for Cadherin 5 has a higher molecular weight of 115kDa due to glycosylation of the protein. 

      The immunogen used to raise this antibody has 89% homology with Cadherin 18, 88kDa , which we believe is the additional observed band at 117kDa, again due to glycosylation of the protein. 

    • Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)
      Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)This image is courtesy of an Abreview submitted by Kara Shumansky

      ab33168 staining VE Cadherin in the endothelial cell line from Human liver by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Paraformaldehyde. Samples were incubated with primary antibody (1/100 in PBS + 2.5% BSA + 0.1% triton) for 1 hour at 37°C. Alexa Fluor 594 Chicken anti-Rabbit IgG (H+L) Cross-Adsorbed Secon was used as the secondary antibody at 4 µg/ml.

      See Abreview

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (155)

    Publishing research using ab33168? Please let us know so that we can cite the reference in this datasheet.

    ab33168 has been referenced in 155 publications.

    • Guo C  et al. Bilobalide reversibly modulates blood-brain barrier permeability through promoting adenosine A1 receptor-mediated phosphorylation of actin-binding proteins. Biochem Biophys Res Commun 526:1077-1084 (2020). PubMed: 32312522
    • Dong W  et al. Mesenchymal-endothelial transition-derived cells as a potential new regulatory target for cardiac hypertrophy. Sci Rep 10:6652 (2020). PubMed: 32313043
    • González-González A  et al. Usefulness of melatonin as complementary to chemotherapeutic agents at different stages of the angiogenic process. Sci Rep 10:4790 (2020). PubMed: 32179814
    • Li X  et al. Function of BMP4 in the Formation of Vasculogenic Mimicry in Hepatocellular Carcinoma. J Cancer 11:2560-2571 (2020). PubMed: 32201526
    • Puech C  et al. Direct oral anticoagulants are associated with limited damage of endothelial cells of the blood-brain barrier mediated by the thrombin/PAR-1 pathway. Brain Res 1719:57-63 (2019). PubMed: 31121158
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-8 of 8 Abreviews or Q&A

    Western blot abreview for Anti-VE Cadherin antibody - Intercellular Junction Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human umbilical vein endothelial cell)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (10%)
    Loading amount
    20 µg
    Treatment
    siRNA for 48h
    Specification
    human umbilical vein endothelial cell
    Blocking step
    Milk as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: ~25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 02 2017

    Western blot abreview for Anti-VE Cadherin antibody - Intercellular Junction Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human umbilical vein endothelial cell)
    Gel Running Conditions
    Non-reduced Denaturing (10% gel)
    Loading amount
    20 µg
    Treatment
    siRNA for 48hrs
    Specification
    human umbilical vein endothelial cell
    Blocking step
    Milk as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: ~25°C
    Read More

    Dr. 振洋 余

    Verified customer

    Submitted Mar 28 2017

    Western blot abreview for Anti-VE Cadherin antibody - Intercellular Junction Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HUVEC)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    HUVEC
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 26 2016

    Western blot abreview for Anti-VE Cadherin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    40 µg
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Sample
    Mouse Cell lysate - whole cell (mouse ips cells)
    Specification
    mouse ips cells
    Treatment
    25ng/ml or 50 ng/ml VEGF for 4 or 6 days
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 12 2013

    Western blot abreview for Anti-VE Cadherin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Non-reduced Denaturing (8% gel)
    Sample
    Human Cell lysate - whole cell (HUVEC)
    Specification
    HUVEC
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 07 2013

    Western blot abreview for Anti-VE Cadherin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Whole Lung)
    Loading amount
    30 µg
    Specification
    Whole Lung
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 05 2012

    Western blot abreview for Anti-VE Cadherin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293)
    Loading amount
    30 µg
    Specification
    HEK293
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 05 2012

    Western blot abreview for Anti-VE Cadherin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Chicken Cell lysate - whole cell (CAM)
    Loading amount
    50 µg
    Specification
    CAM
    Gel Running Conditions
    Reduced Denaturing (8%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 04 2011

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