Overview

  • Product name

    Anti-VE Cadherin (phospho Y685) antibody
    See all VE Cadherin primary antibodies
  • Description

    Rabbit polyclonal to VE Cadherin (phospho Y685)
  • Host species

    Rabbit
  • Specificity

    ab119785 detects a 140 kDa band corresponding to VE cadherin in western blots of Human endothelial cells treated with pervanadate, and this band is not detected in untreated cells or after alkaline phosphatase treatment.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phospho-peptide (Y685), conjugated to carrier protein, corresponding to amino acids surrounding tyrosine 685 in Human VE Cadherin.

  • Positive control

    • Human umbilical vein endothelial cells stimulated with pervanadate.
  • General notes

    Do not aliquot.

Properties

Applications

Our Abpromise guarantee covers the use of ab119785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 140 kDa (predicted molecular weight: 88 kDa).

Target

  • Function

    Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton.
  • Tissue specificity

    Endothelial tissues and brain.
  • Sequence similarities

    Contains 5 cadherin domains.
  • Post-translational
    modifications

    Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB.
  • Cellular localization

    Cell junction. Cell membrane. Found at cell-cell boundaries and probably at cell-matrix boundaries.
  • Information by UniProt
  • Database links

  • Alternative names

    • 7B 4 antibody
    • 7B4 antibody
    • 7B4 antigen antibody
    • CADH5_HUMAN antibody
    • Cadherin 5 antibody
    • Cadherin 5 type 2 antibody
    • Cadherin 5, type 2 (vascular endothelium) antibody
    • Cadherin 5, type 2, VE cadherin (vascular epithelium) antibody
    • cadherin, vascular endothelial antibody
    • cadherin, vascular endothelial, 1 antibody
    • Cadherin-5 antibody
    • Cadherin5 antibody
    • CD 144 antibody
    • CD144 antibody
    • CD144 antigen antibody
    • CDH 5 antibody
    • CDH5 antibody
    • CDH5 protein antibody
    • Endothelial specific cadherin antibody
    • FLJ17376 antibody
    • OTTHUMP00000174777 antibody
    • Vascular endothelial cadherin antibody
    • Vascular epithelium cadherin antibody
    • VE Cad antibody
    • VE-cadherin antibody
    • VEC antibody
    see all

Images

  • All lanes : Anti-VE Cadherin (phospho Y685) antibody (ab119785) at 1/1000 dilution

    Lane 1 : Human umbilical vein endothelial
    cells stimulated with pervanadate (1 mM) for 30 min.
    Lane 2 : Human umbilical vein endothelial
    cells stimulated with pervanadate (1 mM) for 30 min then treated with
    alkaline phosphatase

    Predicted band size: 88 kDa
    Observed band size: 140 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Soni D  et al. Deubiquitinase function of A20 maintains and repairs endothelial barrier after lung vascular injury. Cell Death Discov 4:60 (2018). Read more (PubMed: 29796309) »
  • Lakshmikanthan S  et al. Rap1B promotes VEGF-induced endothelial permeability and is required for dynamic regulation of the endothelial barrier. J Cell Sci 131:N/A (2018). Read more (PubMed: 29222111) »
See all 7 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (placenta)
Gel Running Conditions
Reduced Denaturing (gel: 6% (upper half), 10% (below))
Loading amount
30 µg
Specification
placenta
Blocking step
5% milk + 2% BSA in TBS-T as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Feb 06 2018

Application
Western blot
Sample
Mouse Tissue lysate - whole (lung)
Gel Running Conditions
Reduced Denaturing (6%)
Loading amount
30 µg
Specification
lung
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Sep 09 2016

Answer

Thank you for your inquiry.

I am happy to confirm that both antibodies are tested and guaranteed for human sample in WB.

I would like to assure you that both antibodies are suitable for your purposes.

They are antibodies generated with different rabbits but with the same immunogen.

Since you are working with HUVEC cells and ab27775 is also specifically tested with Huvec cell that were stimulated with VEGF, maybe ab27775 would be the more suitable choice for your experiments.

I hope this information is helpful and wish you good luck with your experiments.

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