Anti-VE Cadherin (phospho Y685) antibody (ab119785)
Key features and details
- Rabbit polyclonal to VE Cadherin (phospho Y685)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-VE Cadherin (phospho Y685) antibody
See all VE Cadherin primary antibodies -
Description
Rabbit polyclonal to VE Cadherin (phospho Y685) -
Host species
Rabbit -
Specificity
ab119785 detects a 140 kDa band corresponding to VE cadherin in western blots of Human endothelial cells treated with pervanadate, and this band is not detected in untreated cells or after alkaline phosphatase treatment. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human VE Cadherin (phospho Y685).
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Positive control
- Human umbilical vein endothelial cells stimulated with pervanadate.
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General notes
Do not aliquot.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab119785 was cross-adsorbed to an unrelated phospho-tyrosine peptide and unphosphorylated VE Cadherin (Y685) peptide before affinity purification using phospho-VE Cadherin (Y685) peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/1000. Detects a band of approximately 140 kDa (predicted molecular weight: 88 kDa).
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Notes |
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WB
1/1000. Detects a band of approximately 140 kDa (predicted molecular weight: 88 kDa). |
Target
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Function
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. -
Tissue specificity
Endothelial tissues and brain. -
Sequence similarities
Contains 5 cadherin domains. -
Post-translational
modificationsPhosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB. -
Cellular localization
Cell junction. Cell membrane. Found at cell-cell boundaries and probably at cell-matrix boundaries. - Information by UniProt
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Database links
- Entrez Gene: 1003 Human
- Entrez Gene: 12562 Mouse
- Entrez Gene: 307618 Rat
- Omim: 601120 Human
- SwissProt: P33151 Human
- SwissProt: P55284 Mouse
- Unigene: 76206 Human
- Unigene: 21767 Mouse
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Alternative names
- 7B 4 antibody
- 7B4 antibody
- 7B4 antigen antibody
see all
Images
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All lanes : Anti-VE Cadherin (phospho Y685) antibody (ab119785) at 1/1000 dilution
Lane 1 : Human umbilical vein endothelial
cells stimulated with pervanadate (1 mM) for 30 min.
Lane 2 : Human umbilical vein endothelial
cells stimulated with pervanadate (1 mM) for 30 min then treated with
alkaline phosphatase
Predicted band size: 88 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (26)
ab119785 has been referenced in 26 publications.
- Kubra KT et al. Tunicamycin Protects against LPS-Induced Lung Injury. Pharmaceuticals (Basel) 15:N/A (2022). PubMed: 35215247
- Nyandwi JB et al. Rosmarinic acid downregulates the oxLDL‑induced interaction between monocytes and endothelial cells, in addition to monocyte diapedesis, under high glucose conditions. Int J Mol Med 49:N/A (2022). PubMed: 35315501
- Liu MM et al. Diammonium glycyrrhizinate lipid ligand ameliorates lipopolysaccharide-induced acute lung injury by modulating vascular endothelial barrier function. Exp Ther Med 21:303 (2021). PubMed: 33717246
- Baganha F et al. Atorvastatin pleiotropically decreases intraplaque angiogenesis and intraplaque haemorrhage by inhibiting ANGPT2 release and VE-Cadherin internalization. Angiogenesis 24:567-581 (2021). PubMed: 33550461
- Su K et al. YAP expression in endothelial cells prevents ventilator-induced lung injury. Am J Physiol Lung Cell Mol Physiol 320:L568-L582 (2021). PubMed: 33565367